Merck
CN
所有图片(1)

文件

安全信息

P3391

Millipore

蛋白 A-琼脂糖™ 来源于金黄色葡萄球菌

lyophilized powder

登录查看公司和协议定价
所有图片(1)
别名:
蛋白 A-琼脂糖
MDL编号:
MFCD00165563
NACRES:
NA.56

形式

lyophilized powder

质量水平

200

包装

glass bottle of 1 g
glass bottle of 1.5 g
glass bottle of 250 mg
glass bottle of 5 g

储存条件

do not freeze

标记范围

3 mg per mL

技术

affinity chromatography: suitable

颜色

white to gray

基质

Sepharose CL-4B

基质活化

cyanogen bromide

基质附着

amino

基质隔离区

1 atom

容量

≥16 mg/mL binding capacity (human IgG)

溶解性

water: 1 g/L at 20 °C

适用性

conforms to structure for swelling capacity (1g swells to 4 to 5 mL)

储存温度

2-8°C

正在寻找类似产品? Visit 产品对比指南

相关类别

一般描述

当与抗体连接时，蛋白A-琼脂糖凝胶有助于提高交联到柱基质上的IgG的抗原结合能力。在这种情况下，结合发生在蛋白A与IgG分子的Fc部分之间，使抗原特异性位点游离。

应用

蛋白A-琼脂糖凝胶™用于亲和层析、抗体纯化和表征、免疫亲和基质、蛋白层析、蛋白A、G和L树脂以及重组蛋白表达和分析。蛋白A-琼脂糖凝胶™已用于制定研究蛋白相互作用的策略，改善乳糜泻检测，以及研究儿童糖尿病。
蛋白A-琼脂糖凝胶具有以下用途：
  • 用于免疫沉淀胎盘，使用蛋白印迹技术进行蛋白质和RNA分析。
  • 用于染色质免疫沉淀，以了解介体和特定启动子染色质结构重构之间的联系。
  • 用作裂解缓冲液，以去除雌性大鼠脑样本蛋白质提取中的内源性G型Ig。
  • 用于兔抗血清的[32P]磷肉豆蔻素C的免疫沉淀反应，以研究蛋白激酶C （PKC）和磷肉豆蔻素C （PMC）在T细胞发育过程中的下调。

数量

膨胀：1 g膨胀至约4 ml。

外形

以用乳糖和葡聚糖稳定的冻干粉形式供应。

法律信息

Sepharose is a trademark of Cytiva

相关产品

产品编号
说明
价格

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

为方便起见，与您过往购买产品相关的文件已保存在文档库中。

访问文档库

难以找到您所需的产品或批次号码？

在网站页面上，产品编号会附带包装尺寸/数量一起显示（例如：T1503-25G）。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).

示例

T1503
货号
-
25G
包装规格/数量

其它示例：

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

遇到问题？欢迎随时联系我们技术服务 寻求帮助

批号可以在产品标签上"批“ （Lot或Batch）字后面找到。

Aldrich 产品

  • 如果您查询到的批号为 TO09019TO 等，请输入去除前两位字母的批号：09019TO。

  • 如果您查询到的批号含有填充代码（例如05427ES-021），请输入去除填充代码-021的批号：05427ES。

  • 如果您查询到的批号含有填充代码（例如 STBB0728K9），请输入去除填充代码K9的批号：STBB0728。

未找到您寻找的产品？

部分情况下，可能未在线提供COA。如果搜索不到COA，可在线索取。

索取COA

  1. How do I swell P3391, Protein A-Sepharose® resin? 

    The lyophilized product should be swollen in buffer A for 30 minutes or longer at room temperature. Do not stir with any kind of mechanical stirrer.  One gram of powder typically swells to 3-4 mL of the hydrated gel.  This, along with other useful information (such as the formulation of buffer A) can be found on the product information sheet (under Documents, above).

  2. How can I store the hydrated P3391, Protein A-Sepharose® resin? 

    Suspensions stored in the refrigerator in the presence of a suitable bacteriostatic agent, such as 20% ETOH, retain IgG binding activity. Another storage option is to put the resin into phosphate buffer saline, pH 7 with about 0.01% preservative (thimerosal or sodium azide).

  3. What can P3391, Protein A-Sepharose® resin tolerate? 

    Protein A Sepharose CL-4B exhibits high chemical and mechanical stability, and is stable over the pH range 2-11. It withstands high concentrations of denaturants, such as urea and guanidine hydrochloride. The gel displays high thermal stability but is not autoclavable. Furthermore, the gel can, if handled with care, be used many times.

  4. How do I clean Product P3391, Protein A-Sepharose®, resin?

    It may be possible to clean the resin by washing it with 10-20 volumes of 100 mM Tris or borate buffer, pH8.5, containing 0.5-2.0 M NaCl, followed by 10-20 volumes of 100 mM acetate buffer, pH 4.0, containing0.5-2.0 M NaCl. Re equilibrate the resin with 20 volumes of buffer A.

  5. What is the binding capacity of Product P3391, Protein A-Sepharose®,  resin for my immunoglobulins?

    A table is available on the product information sheet (under Documents, above).

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

P Hornbeck et al.
Molecular and cellular biology, 9(9), 3727-3735 (1989-09-01)
The regulation and expression of protein kinase C (PKC) and phosphomyristin C (PMC) (a principal substrate of PKC which is the major myristylated protein in lymphocyte and glioma lines that express it) in murine B and T lymphocytes were investigated.
Tracie A Hennen-Bierwagen et al.
Plant physiology, 149(3), 1541-1559 (2009-01-27)
Starch biosynthetic enzymes from maize (Zea mays) and wheat (Triticum aestivum) amyloplasts exist in cell extracts in high molecular weight complexes; however, the nature of those assemblies remains to be defined. This study tested the interdependence of the maize enzymes
M Iezzi et al.
Molecular endocrinology (Baltimore, Md.), 13(2), 202-212 (1999-02-11)
Insulin-secreting cells express four GTPases of the Rab3 family. After separation of extracts of INS-1 cells on a sucrose density gradient, the bulk of the A, B, and C isoforms was recovered in the fractions enriched in insulin-containing secretory granules.
Frédéric Sorgeloos et al.
Journal of virology, 85(7), 3690-3694 (2011-01-14)
The L protein encoded by Theiler's murine encephalomyelitis virus (TMEV) is a unique example of a picornaviral protein encoded by an alternative open reading frame. This protein is an important determinant of TMEV persistence in the mouse central nervous system.
Lionel Gissot et al.
Plant physiology, 142(3), 931-944 (2006-10-10)
The sucrose nonfermenting-1 protein kinase (SNF1)/AMP-activated protein kinase subfamily plays a central role in metabolic responses to nutritional and environmental stresses. In yeast (Saccharomyces cerevisiae) and mammals, the beta- and gamma-noncatalytic subunits are implicated in substrate specificity and subcellular localization
查看所有相关文献

商品

Antibody Fragmentation with Pepsin Digestion

Antibody fragmentation with our pepsin digestion protocol for IgG antibody fragmentation and preparation of F(ab’).

实验方案

Immunoprecipitation Procedure

To determine the molecular weights of protein antigens, to study protein/protein interactions, to determine specific enzymatic activity, to monitor protein post-translational modifications and to determine the presence and quantity of proteins.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门