biological source
protein A from Staphylococcus aureus
description
protein A leaching <= 5 ng/mL
form
aqueous suspension
concentration
50%
matrix
6% Fast-flow beaded agarose
capacity
37-43 mg/mL binding capacity (IgG)
storage temp.
2-8°C
Application
Protein A琼脂糖用于亲和层析、抗体纯化、表征,以及蛋白 A、G 和 L 树脂。Protein A琼脂糖已用于研究蛋白 A 免疫吸附在慢性扩张型心肌病患者中的作用,以及研究多发性硬化和癌症。
Protein A– 琼脂Fast Flow 已用于:
Protein A– 琼脂Fast Flow 已用于:
- 免疫沉淀转染病毒患者正常肾脏和幼鼠肾脏细胞的蛋白质复合物 TRRAP/p400 的
- 从抗血清中纯化多克隆 IgG
- 在 HA-Ano9 质粒转染的人胚胎肾细胞的免疫沉淀分析中
Features and Benefits
- 高容量
- 低泄漏
- 用于纯化单克隆抗体和多克隆抗体的稳定亲和介质
- 非常适合从细胞培养中快速捕获免疫球蛋白
Physical form
悬浮液含 0.01% 硫柳汞
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Boris Gorbatyuk et al.
Molecular microbiology, 55(4), 1233-1245 (2005-02-03)
DnaA protein binds bacterial replication origins and it initiates chromosome replication. The Caulobacter crescentus DnaA also initiates chromosome replication and the C. crescentus response regulator CtrA represses chromosome replication. CtrA proteolysis by ClpXP helps restrict chromosome replication to the dividing
Verena K Hehle et al.
BMC biotechnology, 11, 128-128 (2012-01-03)
Interest in using plants for production of recombinant proteins such as monoclonal antibodies is growing, but proteolytic degradation, leading to a loss of functionality and complications in downstream purification, is still a serious problem. In this study, we investigated the
Henrik Buschmann et al.
Journal of cell science, 128(11), 2033-2046 (2015-04-25)
The preprophase band of microtubules performs the crucial function of marking the plane of cell division. Although the preprophase band depolymerises at the onset of mitosis, the division plane is 'memorized' by a cortical division zone to which the phragmoplast
Spiro Marangoudakis et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 32(30), 10365-10369 (2012-07-28)
Ca(V)2.2 (N-type) calcium channels control the entry of calcium into neurons to regulate essential functions but most notably presynaptic transmitter release. Ca(V)2.2 channel expression levels are precisely controlled, but we know little of the cellular mechanisms involved. The ubiquitin proteasome
Nolan J Hoffman et al.
Molecular metabolism, 41, 101048-101048 (2020-07-02)
Glycogen is a major energy reserve in liver and skeletal muscle. The master metabolic regulator AMP-activated protein kinase (AMPK) associates with glycogen via its regulatory β subunit carbohydrate-binding module (CBM). However, the physiological role of AMPK-glycogen binding in energy homeostasis
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实验方案
A complete workflow for the intact and middle-up mass analysis of reduced and non-reduced monoclonal antibodies based on SEC-MS with sample preparation by protein-A affinity clean-up.
SEC-MS protocol for rapid glycoprofiling of monoclonal antibodies with antibody purification and mass spectrometer calibration.
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