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Merck
CN

P350-05

Porcine Coronary Artery Smooth Muscle Cells: PCASMC (Cryovial)

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关于此项目

NACRES:
NA.81
UNSPSC Code:
41106514
Biological source:
Porcine coronary artery
Growth mode:
Adherent
Morphology:
smooth muscle
Relevant disease(s):
diabetes; stroke; cardiovascular diseases
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biological source

Porcine coronary artery

form

solid

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

karyotype

2n = 38

morphology

smooth muscle

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

diabetes; stroke; cardiovascular diseases

shipped in

dry ice

storage temp.

−196°C

Quality Level

Application

vascular research, supply of blood to heart muscle

Biochem/physiol Actions

Artery

General description

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PCASMC were used to investigate the role of c-Jun in smooth muscle proliferation, and to show that c-Jun inhibition abrogates smooth muscle cell growth and intimal thickening after arterial injury (Khachigian, 2002). PCASMC were also used to develop poly (l-lactide-co-caprolactone) nanofibrous scaffold material with potential for tissue engineering (Dong, 2008).

Characterization: positive for smooth muscle cell specific alpha-actin expression.

Other Notes

Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO

Preparation Note

  • 2nd passage, >500,000 cells in Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO
  • Can be cultured at least 16 doublings
Please refer to the PCASMC Culture Protocol.

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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分析证书(COA)

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In Young Choi et al.
Biomaterials, 269, 120222-120222 (2020-08-02)
Stem cell fate is largely determined by cellular signaling networks and is heavily dependent on the supplementation of exogenous recombinant proteins into culture media; however, uneven distribution and inconsistent stability of recombinant proteins are closely associated with the spontaneous differentiation

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