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文件

P3563

Sigma-Aldrich

磷酸盐缓冲剂，含0.05% TWEEN® 20，pH 7.4

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别名:
1X PBS + 0.05% TWEEN® 20, 1X PBS + 0.05% TWEEN® 20, PBS-T, PBST
eCl@ss:
32129211
NACRES:
NA.25

形式

powder

溶解性

water: soluble, clear, colorless (1 pouch dissolved in 1L)

application(s)

cell analysis
diagnostic assay manufacturing

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相关类别

一般描述

Phosphate Buffered Saline with Tween®-20, or PBST, is primarily used in immunoassays as a washing and blocking buffer. The phosphate buffer maintains a stable pH 7.4, suitable for many biological reactions and assays. The salt or saline solution in PBS-T provides the necessary ionic strength for optimal protein solubility and stability. The addition of 0.05% TWEEN®-20, a non-ionic detergent, helps reduce background signals by blocking non-specific binding sites on proteins. PBST buffer enhances the washing efficiency by removing unwanted substances and limits non-specific antibody binding sites, while preserving the integrity of the target proteins.

应用

磷酸盐缓冲盐水已被用于：
  • 酶联免疫吸附测定（ELISA）
  • 端粒-FISH（Tel-FISH）和端粒/免疫染色-FISH（Teli-Fish）
  • 细胞分离

包装

锡箔袋包装

重悬

Contents of one pouch dissolved in 1 L dH2O or ultrapure water to yield 0.01 M phosphate buffered saline, 0.138M NaCl, 0.0027M KCl, and 0.05% Tween 20, pH7.4.

其他说明

For additional information on our range of Biochemicals, please complete this form.

法律信息

TWEEN is a registered trademark of Croda International PLC

储存分类代码

13 - Non Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

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示例

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ELISA Procedures

ELISA protocols providing detailed instructions on performing indirect ELISA and Capture ELISA . Learn more about recommended products and techniques for performing both sandwich ELISA and indirect ELISA experiments.

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