Merck
CN

P3563

Sigma-Aldrich

磷酸盐缓冲剂,含0.05% TWEEN® 20,pH 7.4

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别名:
1X PBS + 0.05% TWEEN® 20, 1X PBS + 0.05% TWEEN® 20, PBS-T, PBST
eCl@ss:
32129211
NACRES:
NA.25

形式

powder

质量水平

溶解性

water: soluble, clear, colorless (1 pouch dissolved in 1L)

application(s)

diagnostic assay manufacturing

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solubility

water: soluble, clear, colorless (1 pouch dissolved in 1L)

solubility

water: soluble

solubility

water: soluble

solubility

water: soluble

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

一般描述

Phosphate Buffered Saline with Tween®-20, or PBST, is primarily used in immunoassays as a washing and blocking buffer. The phosphate buffer maintains a stable pH 7.4, suitable for many biological reactions and assays. The salt or saline solution in PBS-T provides the necessary ionic strength for optimal protein solubility and stability. The addition of 0.05% TWEEN®-20, a non-ionic detergent, helps reduce background signals by blocking non-specific binding sites on proteins. PBST buffer enhances the washing efficiency by removing unwanted substances and limits non-specific antibody binding sites, while preserving the integrity of the target proteins.

应用

磷酸盐缓冲盐水已被用于:
  • 酶联免疫吸附测定(ELISA)
  • 端粒-FISH(Tel-FISH)和端粒/免疫染色-FISH(Teli-Fish)
  • 细胞分离

包装

锡箔袋包装

重悬

Contents of one pouch dissolved in 1 L dH2O or ultrapure water to yield 0.01 M phosphate buffered saline, 0.138M NaCl, 0.0027M KCl, and 0.05% Tween 20, pH7.4.

法律信息

TWEEN is a registered trademark of Croda International PLC

储存分类代码

13 - Non Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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示例

T1503
货号
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25G
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实验方案

ELISA 操作步骤

ELISA实验方案提供了进行间接ELISA和捕获ELISA的详细说明。了解进行夹心式ELISA和间接ELISA实验的推荐产品和技术信息。

ELISA Procedures

ELISA protocols providing detailed instructions on performing indirect ELISA and Capture ELISA . Learn more about recommended products and techniques for performing both sandwich ELISA and indirect ELISA experiments.

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