表单
powder
比活
≥0.4 unit/mg solid
分子量
58 kDa by gel filtration
储存温度
−20°C
SMILES字符串
O(CC(O)C)CC#C
InChI
1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3
InChI key
GZCWLCBFPRFLKL-UHFFFAOYSA-N
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应用
酸性磷酸酶（APase）可非特异性地催化磷酸单酯和酸酐的水解以产生无机磷酸盐。它可用于研究磷酸盐的生产、转运和再循环，以及细胞的代谢和能量转导过程。 产品P3627来自于小麦胚芽，已被用于确定磷酸酶处理对3F3/2染色的影响。
生化/生理作用
植物酸性磷酸酶（APase）位于细胞溶质、液泡和细胞壁中。植物酸性磷酸酶对于磷酸盐的获取非常重要，其可以调动土壤中的有机磷酸盐。 磷酸盐饥饿可诱导酸性磷酸酶的产生。 酸性磷酸酶起源于发芽过程中的糊粉和黄芩组织。 它可以水解植酸、ATP、蛋白质磷酸和核苷酸磷酸。 它可在一般代谢反应中起作用。 小麦胚胎APase由四种同工酶组成。 小麦胚芽APase的分子量为58 kDa（凝胶过滤），最适pH值为5.7，pH范围为4.0-7.0，最适温度为45°C。
制备说明
Prepared essentially by method of Singer, T.P., J. Biol.Chem., 174, 11 (1948).
分析说明
含有脂肪酶
其他说明
在pH 4.8，37 °C条件下，一个单位可在每分钟水解1.0 μmole 对硝基苯磷酸盐。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
此项目有
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实验方案
在测定酸性磷酸酶活性时，本操作流程使用对硝基苯基磷酸盐进行比色法测定。酶活性是使用分光光度计在410 nm处测定的。
Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)
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