P3627
酸性磷酸酶 来源于小麦胚芽
≥0.4 unit/mg solid
别名:
酸性磷酸酶 来源于小麦胚芽, （正磷酸单酯磷酸水解酶[酸最适]）
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关于此项目
化学文摘社编号:
eCl@ss:
42010102
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-630-9
MDL number:
Specific activity:
≥0.4 unit/mg solid
SMILES string
O(CC(O)C)CC#C
InChI
1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3
InChI key
GZCWLCBFPRFLKL-UHFFFAOYSA-N
form
powder
specific activity
≥0.4 unit/mg solid
mol wt
58 kDa by gel filtration
storage temp.
−20°C
Quality Level
Application
酸性磷酸酶（APase）可非特异性地催化磷酸单酯和酸酐的水解以产生无机磷酸盐。它可用于研究磷酸盐的生产、转运和再循环，以及细胞的代谢和能量转导过程。 产品P3627来自于小麦胚芽，已被用于确定磷酸酶处理对3F3/2染色的影响。
Biochem/physiol Actions
植物酸性磷酸酶（APase）位于细胞溶质、液泡和细胞壁中。植物酸性磷酸酶对于磷酸盐的获取非常重要，其可以调动土壤中的有机磷酸盐。 磷酸盐饥饿可诱导酸性磷酸酶的产生。 酸性磷酸酶起源于发芽过程中的糊粉和黄芩组织。 它可以水解植酸、ATP、蛋白质磷酸和核苷酸磷酸。 它可在一般代谢反应中起作用。 小麦胚胎APase由四种同工酶组成。 小麦胚芽APase的分子量为58 kDa（凝胶过滤），最适pH值为5.7，pH范围为4.0-7.0，最适温度为45°C。
Preparation Note
Prepared essentially by method of Singer, T.P., J. Biol.Chem., 174, 11 (1948).
Analysis Note
含有脂肪酶
Other Notes
在pH 4.8，37 °C条件下，一个单位可在每分钟水解1.0 μmole 对硝基苯磷酸盐。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
Zuzana Novotná et al.
FEBS letters, 554(1-2), 50-54 (2003-11-05)
Phospholipase D (PLD) forms the major family of phospholipases that was first discovered and cloned in plants. In this report we have shown, for the first time, that C2 phosphatidylinositol-4,5-bisphosphate (PIP2)-dependent PLD(s) from 5 day hypocotyls of Brassica oleracea associated
H G Yu et al.
The Journal of cell biology, 145(3), 425-435 (1999-05-04)
We have identified a maize homologue of yeast MAD2, an essential component in the spindle checkpoint pathway that ensures metaphase is complete before anaphase begins. Combined immunolocalization of MAD2 and a recently cloned maize CENPC homologue indicates that MAD2 localizes
Yueming Liang et al.
Frontiers in microbiology, 11, 571209-571209 (2020-12-18)
phoD-harboring microorganisms facilitate mineralization of organic phosphorus (P), while their role in the regulation of soil P turnover under P-limited conditions in Pinus massoniana plantations is poorly understood. The aim of the present study was to investigate the effects of
Jakob Madsen Pedersen et al.
PLoS genetics, 8(12), e1003128-e1003128 (2013-01-04)
To investigate the role of DNA topoisomerases in transcription, we have studied global gene expression in Saccharomyces cerevisiae cells deficient for topoisomerases I and II and performed single-gene analyses to support our findings. The genome-wide studies show a general transcriptional
M V Stogov et al.
Klinicheskaia laboratornaia diagnostika, (11)(11), 21-22 (2013-01-12)
The article deals with the results of study of chemical composition of solitary cysts and blood serum of 27 patients. The results demonstrated that qualitative composition of f content of unicameral bone cysts is identical to chemical composition of blood
实验方案
Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)
在测定酸性磷酸酶活性时，本操作流程使用对硝基苯基磷酸盐进行比色法测定。酶活性是使用分光光度计在410 nm处测定的。
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