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P3738

Protein Kinase G Iβ human

Name: Protein Kinase G Iβ human
Brand: SIGMA

≥95% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf9 cells, buffered aqueous glycerol solution

别名:

cyclic-Guanosine Monophosphate-dependent Protein Kinase 1β human

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关于此项目

UNSPSC Code:

12352204

NACRES:

NA.54

EC 号:

2.7.11.12 (BRENDA, IUBMB)

MDL number:

MFCD03458230

Specific activity:

≥1.5 units/mg protein (20-fold stimulation by cGMP (5 μM))

Assay:

≥95% (SDS-PAGE)

Recombinant:

expressed in baculovirus infected Sf9 cells

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属性

recombinant

expressed in baculovirus infected Sf9 cells

Quality Segment

200

assay

≥95% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

≥1.5 units/mg protein (20-fold stimulation by cGMP (5 μM))

mol wt

76 kDa (monomer)

UniProt accession no.

Q13976

relevant disease(s)

cancer

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... PRKG1(5592)

说明

Application

Protein Kinase G is a serine/threonine-specific protein kinase that is activated by cGMP. Protein Kinase G Iβ is used to induce apoptosis and inhibit cell proliferation.

Biochem/physiol Actions

Protein Kinase G Iβ induces apoptosis in certain cell lines such as human breast cancer cell lines MCF-7 and MDA-MB-468. It inhibits cell proliferation and induces apoptosis in colon cancer cell lines.

Physical form

Solution in 20 mM Tris buffer, pH 7.4, 1 mM EDTA, 1 mM β-mercaptoethanol, 100 mM NaCl, 10 U/ml Trasylol, and 50% glycerol.

Other Notes

One unit will phosphorylate 1 micromole of VASPtide(RRKVSKQE) substrate per minute in 10 mM HEPES, pH 7.4, 5 mM MgCl2, 1 mM DTE and 0.2 mM EDTA.

存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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全球贸易项目编号

货号	GTIN
P3738-5UG	04061838071859