P3804
邻苯二胺 二盐酸盐
chromogenic, tablet
别名:
1,2-Phenylenediamine tablet, OPD, OPD Tablet
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关于此项目
线性分子式:
C6H4(NH2)2·2HCl
化学文摘社编号:
分子量:
181.06
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
210-418-7
MDL number:
Beilstein/REAXYS Number:
3912045
产品名称
邻苯二胺 二盐酸盐, tablet, 5 mg substrate per tablet
Quality Segment
form
tablet
mp
258 °C (dec.) (lit.)
solubility
water: 1 tablet/10 mL, clear, colorless
storage temp.
2-8°C
SMILES string
Cl[H].Cl[H].Nc1ccccc1N
InChI
1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
InChI key
RIIWUGSYXOBDMC-UHFFFAOYSA-N
General description
邻苯二胺二盐酸盐（OPD）是一种显色底物，因为利用辣根过氧化物酶缀合物，因此可用于酶联免疫吸附试验（ELISA）程序。底物产生可溶的最终产物，其颜色为橙棕色，并且可以在450 m下通过分光光度计读取。可以用3N HCl或3M H2SO4终止OPD反应，并在492 nm处读取。
Application
o-Phenylenediamine dihydrochloride has been used:
- as a chromogenic substrate in indirect enzyme-linked immunosorbent assay (ELISA) to detect immunoglobin G (IgG) antibodies against certain recombinant antigens/proteins
- as a streptavidin-horseradish peroxidase (HRP) substrate sandwich ELISA to analyze human interleukin (IL)-17A, IL-23, and IP-10
- as a substrate in ELISA for determination of IgG-class antibodies to Mycobacterium bovis in serum
Packaging
16 mg total tablet weight
Preparation Note
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use.
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Warning
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3