P4056
Platelet-Derived Growth Factor-BB 来源于大鼠
≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
别名:
PDGF-BB
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关于此项目
Biological source:
rat
Recombinant:
expressed in E. coli
Assay:
≥97% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
~30 kDa
Impurities:
≤1 EU/μg
产品名称
Platelet-Derived Growth Factor-BB 来源于大鼠, PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
biological source
rat
recombinant
expressed in E. coli
assay
≥97% (SDS-PAGE)
form
lyophilized powder
potency
1.62 ng/mL EC50
quality
endotoxin tested
mol wt
~30 kDa
packaging
pkg of 5x50 μg, pkg of 50 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μg
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
rat ... Pdgfb(24628)
General description
Platelet-derived growth factor (PDGF) is a 30 kDa cationic polypeptide and consists of two disulphide linked chains A and B. PDGF is expressed as hetero and homodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD.
Application
Platelet-Derived Growth Factor-BB from rat has been used as a growth supplement in mouse embryonic fibroblast culture, motoneuron-muscle co-culture and adult spinal cord neurons culture.
Biochem/physiol Actions
Platelet-derived growth factor (PDGF) elicits multifunctional actions with a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells, and chondrocytes. PDGF is a potent chemoattractant and activator of neutrophils, monocytes, and fibroblasts. PDGF activates smooth muscle relocalization and multiplication. PDGF mediated increased smooth muscle muscle proliferation might be associated with gene regulation. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins, and modulates LDL (low density lipoprotein) receptor binding.
Physical form
Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 2.5 mg bovine serum albumin.
Analysis Note
The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.
Other Notes
Dimer of the B chain of rat PDGF.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
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Platelet-Derived Growth Factor?BB Transactivates the Fibroblast Growth Factor Receptor to Induce Proliferation in Human Smooth Muscle Cells.
Millette E, et al.
Trends in Cardiovascular Medicine, 16.1, 25-28 (2006)
Eileen M Bauer et al.
PloS one, 9(5), e96720-e96720 (2014-05-20)
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Das M, et al.
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商品
Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.
血小板衍生生长因子是在哺乳动物血清中发现的主要有丝分裂原，在血栓形成过程中从血小板释放。
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