P4300
果胶酶 来源于根霉菌 属
suitable for plant cell culture, BioReagent, crude powder
别名:
聚-(1,4-α-D-半乳糖醛酸苷)聚糖水解酶, 聚半乳糖醛酸酶
产品线
BioReagent
表单
crude powder
比活
400-800 units/g solid
技术
cell culture | plant: suitable
储存温度
−20°C
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一般描述
Pectinase P4300 is a natural pectinase (polygalacturonase) derived from fungus Rhizops sp. that randomly hydrolyzes the (1→4)-α-D-galactosiduronic linkages in pectate and other galacturonans. This enzyme breaks down pectin found in the middle lamella of plant cell walls. Pectinase helps facilitate the maceration of plant materials and extraction processes. Pectinase P4300 is used in plant protoplast preparation to digest cell wall prior to organelle isolation.
应用
用于植物原生质体制备，在细胞器分离前消化细胞壁。
其他说明
One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
Jeffrey A Mertens et al.
Fungal genetics and biology : FG & B, 45(12), 1616-1624 (2008-10-22)
A search of the recently sequenced Rhizopus oryzae strain 99-880 genome database uncovered 18 putative polygalacturonase genes with two genes being identical and only one with similarity to a previously reported R. oryzae polygalacturonase gene. The 17 different genes share
Katsuichi Saito et al.
Microbiological research, 159(1), 83-86 (2004-05-27)
The pectinolytic enzyme from the solid-state culture of Rhizopus oryzae NBRC 4707 was purified to homogeneity by column chromatography on CM-Toyopearl 650 M and hydroxylapatite. The molecular weight of the enzyme was estimated by SDS-polyacrylamide gel electrophoresis to be 31,000
Shigenobu Yoshida et al.
Mycological research, 108(Pt 12), 1407-1414 (2005-03-11)
The polygalacturonase (PG)-encoding gene (rpg1) of Rhizopus oryzae, the causal pathogen of rhizopus rot of mulberry, was cloned and sequenced. PGs were partially purified from incubation mixture of 2% pectin medium and their N-terminal amino acid sequences were determined by
K Saito et al.
Journal of industrial microbiology & biotechnology, 30(7), 440-444 (2003-07-05)
Rhizopus oryzae strain NBRC 4707 produced lactic acid and ethanol more efficiently than strain NRRL 395 in potato pulp, an agricultural by-product of the starch industry. The two strains developed comparable activities of xylanase, cellulase, alpha-amylase, and glucoamylase, while the
Henri G Gerken et al.
Planta, 237(1), 239-253 (2012-09-27)
Cell walls of microalgae consist of a polysaccharide and glycoprotein matrix providing the cells with a formidable defense against its environment. We characterized enzymes that can digest the cell wall and weaken this defense for the purpose of protoplasting or
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