P4439
碱性磷酸酶 来源于猪肾脏
lyophilized powder, ≥100 DEA units/mg protein
别名:
Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
eCl@ss:
42010105
EC Number:
232-631-4
NACRES:
NA.54
MDL number:
Specific activity:
≥100 DEA units/mg protein
form
lyophilized powder
specific activity
≥100 DEA units/mg protein
composition
Protein, ≥50%
storage temp.
−20°C
Quality Level
Application
碱性磷酸酶可与抗体和其他蛋白质偶联，用于ELISA、蛋白质免疫印迹和组织化学检测。 碱性磷酸酶也可用于防止DNA自身连接和放射性标记。
Biochem/physiol Actions
碱性磷酸酶是一种水解酶，能使核苷酸、蛋白质和生物碱等分子去磷酸化。
Packaging
包装规格取决于DEA单位
Physical form
从Tris-HCl缓冲液中冻干，含氯化镁和氯化锌，pH 8.0
Analysis Note
通过缩二脲法测定的蛋白质。
Other Notes
在pH 9.8和37℃条件下，1.0个单元每分钟水解1 μmol对硝基苯基磷酸盐。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
Sequencing end-labeled DNA with base-specific chemical cleavages.
A M Maxam et al.
Methods in enzymology, 65(1), 499-560 (1980-01-01)
Naomi Ofer et al.
The Journal of biological chemistry, 288(16), 11287-11293 (2013-03-09)
Protein degradation via prokaryotic ubiquitin-like protein (Pup) tagging is conserved in bacteria belonging to the phyla Actinobacteria and Nitrospira. The physiological role of this novel proteolytic pathway is not yet clear, although in Mycobacterium tuberculosis, the world's most threatening bacterial
Gouri Chaudhuri et al.
Indian journal of biochemistry & biophysics, 50(1), 64-71 (2013-04-27)
The hydrolysis of p-nitrophenyl phosphate (pNPP) by calf intestinal alkaline phosphatase (CIAP) was investigated with respect to kinetic parameters such as V(max), K(m) and K(cat) under varying pH, buffers, substrate concentration, temperature and period of incubation. Highest activity was obtained
George C Ziobro et al.
Journal of food protection, 76(5), 892-898 (2013-05-07)
Over the past 80 years, a variety of methods have been developed to detect underpasteurized or improperly pasteurized milks used in dairy products. Existing methods are hampered by duration of analysis, poor reproducibility, and in some cases the use of
I V Zverinskiĭ et al.
Biomeditsinskaia khimiia, 59(1), 90-96 (2013-05-09)
On the eighth day after ligation of the common bile duct in rats a significant increase in the serum content of total lipids, cholesterol bilirubin and ALT, alkaline phosphatase, and gamma-glutamyltransferase was observed. In the microsomal fraction there was a
实验方案
Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)
碱性磷酸酶的酶法测定，二乙醇胺法（EC 3.1.3.1)
相关内容
QC Methods
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