P4439
碱性磷酸酶 来源于猪肾脏
lyophilized powder, ≥100 DEA units/mg protein
别名:
Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
应用
碱性磷酸酶可与抗体和其他蛋白质偶联，用于ELISA、蛋白质免疫印迹和组织化学检测。 碱性磷酸酶也可用于防止DNA自身连接和放射性标记。
生化/生理作用
碱性磷酸酶是一种水解酶，能使核苷酸、蛋白质和生物碱等分子去磷酸化。
包装
包装规格取决于DEA单位
外形
从Tris-HCl缓冲液中冻干，含氯化镁和氯化锌，pH 8.0
分析说明
通过缩二脲法测定的蛋白质。
其他说明
在pH 9.8和37℃条件下，1.0个单元每分钟水解1 μmol对硝基苯基磷酸盐。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Sequencing end-labeled DNA with base-specific chemical cleavages.
A M Maxam et al.
Methods in enzymology, 65(1), 499-560 (1980-01-01)
Peter M Krawitz et al.
American journal of human genetics, 92(4), 584-589 (2013-04-09)
Recently, mutations in genes involved in the biosynthesis of the glycosylphosphatidylinositol (GPI) anchor have been identified in a new subclass of congenital disorders of glycosylation (CDGs) with a distinct spectrum of clinical features. To date, mutations have been identified in
Gouri Chaudhuri et al.
Indian journal of biochemistry & biophysics, 50(1), 64-71 (2013-04-27)
The hydrolysis of p-nitrophenyl phosphate (pNPP) by calf intestinal alkaline phosphatase (CIAP) was investigated with respect to kinetic parameters such as V(max), K(m) and K(cat) under varying pH, buffers, substrate concentration, temperature and period of incubation. Highest activity was obtained
C Cavallo et al.
Journal of biological regulators and homeostatic agents, 27(1), 165-175 (2013-03-16)
Bone marrow is one of the best characterized stem cell microenvironments that contains Mesenchymal Stem Cells (MSCs), a rare population of non-hematopoietic stromal cells. MSCs have been indicated as a new option for regenerative medicine because of their ability to
Insulin affects dopamine metabolism in Drosophila females under normal and stress conditions.
N V Adonyeva et al.
Doklady. Biochemistry and biophysics, 448, 40-42 (2013-03-13)
实验方案
Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)
碱性磷酸酶的酶法测定，二乙醇胺法（EC 3.1.3.1)
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