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P4707

Poly-ʟ-Lysine Hydrobromide

Name: Poly-ʟ-Lysine Hydrobromide
Brand: SIGMA

synthetic, liquid, 0.01%, suitable for cell culture

别名:

PLL 溶液

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关于此项目

化学文摘社编号:

25988-63-0

UNSPSC Code:

12352202

eCl@ss:

32160406

NACRES:

NA.75

MDL number:

MFCD00165424

Biological source:

synthetic

Form:

liquid

Technique(s):

cell culture | mammalian: suitable

Concentration:

0.01%

Mol wt:

70—150 kDa

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产品名称

多聚-L-赖氨酸溶液, 0.01%, sterile-filtered, BioReagent, suitable for cell culture

biological source

synthetic

Quality Level

300

sterility

sterile-filtered

product line

BioReagent

form

liquid

mol wt

70—150 kDa

packaging

pkg of 50 mL

concentration

0.01%

technique(s)

cell culture | mammalian: suitable

surface coverage

4 μg/cm²

impurities

endotoxin, tested

solubility

water: soluble

shipped in

ambient

storage temp.

2-8°C

SMILES string

N([C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O)C(=O)[C@@H](N)CCCCN

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

InChI key

WBSCNDJQPKSPII-KKUMJFAQSA-N

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General description

聚-L-赖氨酸是一种带正电荷的氨基酸聚合物，每个赖氨酸残基约有一个HBr。氢溴酸盐可使聚-L-赖氨酸以可溶于水的结晶形式存在。在β结构中可能会发现少量的产物，因为HBr干扰氨基与羧基或含N或O部分之间的氢键。

Application

聚-L-赖氨酸涂覆的玻璃盖玻片已用于对用于扫描电子显微镜的样本进行封片。
聚-L-赖氨酸涂覆的孔用于在电极上对PC-12细胞进行捕获。
聚-L-赖氨酸涂覆的硼硅酸盐玻片用于接种病毒载体转导的细胞。

聚-L-赖氨酸聚合物可用于促进细胞对固体底物的粘附、与甲氨蝶呤结合以增加药物转运、胰岛微囊化、细胞微囊化技术、微阵列玻片涂层和染色体制剂。较低分子量的聚-L-赖氨酸（30000-70000）在溶液中粘度较低，但分子量较高的聚赖氨酸在每个分子中提供更多的附着位点。

Biochem/physiol Actions

聚-L-赖氨酸是一种非特异性的细胞附着因子，通过增强细胞膜负离子与培养基表面的静电相互作用，促进细胞对固体基质的粘附。当其吸附到细胞培养表面时，聚-L-赖氨酸的作用是增加可用于细胞结合的带正电荷位点的数量。对于可降解聚-L-赖氨酸的细胞，应采用聚-D-赖氨酸作为附着因子。

Preparation Note

该产品是以溶于水的0.01%无菌过滤溶液形式提供的。用该溶液进行涂覆的玻片应当随后进行一步5分钟的孵育并在室温对其进行干燥。涂覆的玻片在免受灰尘的保护下可稳定一年。

Other Notes

存储类别

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

历史批次信息供参考:

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实验方案

Poly-L-Lysine Cell Attachment Protocol

Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.

多聚-L-赖氨酸细胞附着实验方案

使用多聚-赖氨酸将细胞粘附在固相基质上，多聚-赖氨酸可增强细胞膜表面的负电荷离子与培养表面之间的静电相互作用。

全球贸易项目编号

货号	GTIN
P4707-50ML	04061835569151

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