Merck
CN

P5282

Sigma-Aldrich

鬼笔环肽,异硫氰酸荧光素标记

sequence Amanita phalloides(synthetic: peptide sequence)

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别名:
鬼笔环肽-FITC
经验公式(希尔记法):
C56H60N10O15S2
分子量:
1177.26
MDL编号:
NACRES:
NA.32

生物来源

sequence from Amanita phalloides (synthetic: peptide sequence)

质量水平

形式

solid

荧光

λex 495 nm; λem 520 nm(lit.)

储存温度

−20°C

一般描述

鬼笔环肽是由死亡之帽鬼笔鹅膏产生的鬼笔毒素。它是一种可与肌动蛋白相互作用的环肽,最早是在鬼笔环肽中毒的大鼠体内发现的。它是一种七肽,本质上是环状的,在6位色氨酸和3位半胱氨酸之间具有交联键。鬼笔环肽中氨基酸7(γ-δ-二羟基亮氨酸)的侧链可被修饰,通过该修饰可以产生荧光标记的鬼笔环肽化合物。

应用

异硫氰酸荧光素标记的鬼笔环肽可用于:
  • 观察内皮素-1(ET-1)和血管紧张素II(Ang-II)处理后原代乳鼠心肌细胞(PNCM)和H9C2细胞(大鼠心肌成肌细胞)F-肌动蛋白的重组情况。
  • 在免疫化学中标记微丝。
  • 在免疫荧光分析中对F-肌动蛋白进行染色。

生化/生理作用

鬼笔环肽与聚合肌动蛋白相互作用,而不与寡聚或单体形式相互作用。这种相互作用产生高度稳定的肌动蛋白丝,能抵抗解聚和分解。在大鼠中,这种毒素可导致肝出血死亡,细胞显示出异常的肌动蛋白聚集。对荧光标记的鬼笔环肽化合物进行衍生化后,鬼笔环肽对肌动蛋白的亲和力并未显著改变改变。这些化合物可用于研究真核细胞内肌动蛋白的结构和组织。
结合聚合F肌动蛋白的毒素,从而稳定肌动蛋白并干扰富含肌动蛋白的结构的功能。

象形图

Skull and crossbones

警示用语:

Danger

危险声明

危险分类

Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral

储存分类代码

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

高风险级别生物产品--毒素类产品

分析证书(COA)

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示例

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货号
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包装规格/数量

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How do I dissolve Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?

    This product is soluble in methanol, ethanol, butanol and pyridine. It is soluble in cold water at a concentration of 5 mg/mL; it is much more soluble in hot water. See: The chemicals encyclopedia published by the Royal Society of Chemistry, 12th ed., entry# 7336 (1996). Solutions should be prepared fresh, and protected from light whenever possible.

  6. How do I prepare stock solutions of Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?

    Stock solutions of  phalloidin conjugates have been made in methanol or DMSO at 0.1 to 5 mg/mL. Make final dilutions in aqueous physiological buffers for a staining range from 0.1 μM to 100 μM with corresponding incubation times of 15 minutes to 72 hours.

  7. What are the excitation and emission wavelengths used for fluorescent detection of Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?

    Excitation wavelengths of 540-545 nm and emission wavelengths of 570-573 nm can be used. See: Faulstich, H., J. Muscle Res. Cell Motility, 9, 370 (1988), and Waggoner, A. et al., Methods in Cell Biology, 30, 449 (1989).

  8. How do I stain cells with Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?

    A typical application for staining cells can be found in the product information sheet (under Documents, above).

  9. How do I store Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?

    The product should be stored at a powder in the freezer at -20°C.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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