P5400
果胶酯酶 来源于橘皮
lyophilized powder, ≥150 units/mg protein
别名:
Pectin methylesterase, 果胶甲酯酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-807-0
MDL number:
Specific activity:
≥150 units/mg protein
form
lyophilized powder
specific activity
≥150 units/mg protein
composition
Protein, 20-50% biuret
greener alternative product characteristics
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
greener alternative category
, Enabling
storage temp.
2-8°C
Quality Level
相关类别
General description
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。该产品已用作替代能源的酶。更多信息，请参阅 替代能源的酶研究
Application
在测定沙棘果汁中果胶甲酯酶活性时，使用 Sigma 酶作为标准。它被用于细胞壁果胶的水解，同时研究 拟南芥中的叶状结构 。它也被用于催化果胶水解（使用超声波测量装置），以监测生物过程。
果胶酯酶用于将果胶的甲酯水解为果胶酯和甲醇。含有果胶甲酯酶和果胶果胶水解酶。产品 P5400 来自橘皮，为冻干粉。包含 (NH4)2SO4 和氯化钠。已用于果胶甲基酯酶基因的研究 。
Biochem/physiol Actions
果胶酯酶催化果胶的甲酯水解产生果胶和甲醇。
Physical form
含 (NH4)2SO4 和氯化钠
Analysis Note
由缩二脲测定的蛋白质。
Other Notes
在 pH 7.5、30°C 条件下，1 个单位每分钟从果胶中释放 1.0 μg 酸。
查看更多关于 复合糖分析用酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
Kerstin Müller et al.
Plant physiology, 161(1), 305-316 (2012-11-07)
The methylesterification status of cell wall homogalacturonans, mediated through the action of pectin methylesterases (PMEs), influences the biophysical properties of plant cell walls such as elasticity and porosity, important parameters for cell elongation and water uptake. The completion of seed
Mélanie L'Enfant et al.
International journal of biological macromolecules, 132, 1140-1146 (2019-04-13)
The discovery of molecules that can inhibit the action of phytopathogens is essential to find alternative to current pesticides. Pectin methylesterases (PME), enzymes that fine-tune the degree of methylesterification of plant cell wall pectins, play a key role in the
Daniele Del Corpo et al.
Molecular plant pathology, 21(12), 1620-1633 (2020-10-09)
Pectin is synthesized in a highly methylesterified form in the Golgi cisternae and partially de-methylesterified in muro by pectin methylesterases (PMEs). Arabidopsis thaliana produces a local and strong induction of PME activity during the infection of the necrotrophic fungus Botrytis
Ida Barbara Reca et al.
Plant molecular biology, 79(4-5), 429-442 (2012-05-23)
A pectin methylesterase inhibitor (SolyPMEI) from tomato has been identified and characterised by a functional genomics approach. SolyPMEI is a cell wall protein sharing high similarity with Actinidia deliciosa PMEI (AdPMEI), the best characterised inhibitor from kiwi. It typically affects
Yuri L Dorokhov et al.
PLoS pathogens, 8(4), e1002640-e1002640 (2012-04-13)
Many plants release airborne volatile compounds in response to wounding due to pathogenic assault. These compounds serve as plant defenses and are involved in plant signaling. Here, we study the effects of pectin methylesterase (PME)-generated methanol release from wounded plants
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