P5998
Monoclonal Anti-hPlk1 (Polo Like Kinase 1) antibody produced in mouse
~2 mg/mL, clone 35-206, purified immunoglobulin, buffered aqueous solution
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关于此项目
Conjugate:
unconjugated
Clone:
35-206, monoclonal
Application:
ARR, ICC, IP, WB
Citations:
11
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
35-206, monoclonal
form
buffered aqueous solution
species reactivity
human, rat, mouse
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, microarray: suitable, western blot: 0.04-0.08 μg/mL using whole extract of HeLa cell line
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PLK1(5347)
mouse ... Plk1(18817)
rat ... Plk1(25515)
General description
Monoclonal Anti- hPlk1 (Polo Like Kinase 1) (mouse IgG2b isotype) is derived from the 35-206 hybridoma produced by the fusion of mouse myeloma cells (PAI) and splenocytes from BALB/c mice immunized with human full length Plk1. Polo-like kinases (Plks) are a family of serine/threonine kinases. The Plk family is structurally and functionally related to the polo gene in Drosophila. Plk1 localizes during mitosis in centrosomes, kinetochores and in the central spindle.
Immunogen
human full length Plk1.
Application
Monoclonal Anti-hPlk1 (Polo Like Kinase 1) antibody produced in mouse has been used in:
- enzyme linked immunosorbent assay (ELISA)
- immunoblotting
- immunoprecipitation
- immunocytochemistry
- immunofluorescence staining
Biochem/physiol Actions
PLK1 is a serine-threonine kinase that is expressed in mitotic cells and cancers. This kinase regulates mitotic entry by modulating cdc2/cyclin B and is also involved in Nlp-mediated nucleation of microtubules .
Polo Like Kinase 1 (Plk1) has a role in tumor progression. Plk1 is a target of the DNA damage checkpoint on the basis that its activity is inhibited by DNA damage in G2 and mitosis. Moreover, Plk1 was found to bind to the sequence-specific DNA binding domain of p53. This interaction between p53 and Plk1 inhibits the transcriptional activation and the proapoptotic functions of p53.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
低风险生物材料
常规特殊物品
此项目有
Association of spindle midzone particles with polo-like kinase 1 during meiosis in mouse and human oocytes
Otsuki J, et al.
Reproductive Biomedicine Online, 18(4), 522-528 (2009)
Polo-like kinase 1 (Plk1) inhibits p53 function by physical interaction and phosphorylation
Ando K, et al.
The Journal of biological chemistry, 279(24), 25549-25561 (2004)
Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis
Neef R, et al.
The Journal of cell biology, 162(5), 863-876 (2003)
The crystal structure of the human polo-like kinase-1 polo box domain and its phospho-peptide complex
Cheng KY, et al.
The Embo Journal, 22(21), 5757-5768 (2003)
Martina Casenghi et al.
Developmental cell, 5(1), 113-125 (2003-07-11)
In animal cells, most microtubules are nucleated at centrosomes. At the onset of mitosis, centrosomes undergo a structural reorganization, termed maturation, which leads to increased microtubule nucleation activity. Centrosome maturation is regulated by several kinases, including Polo-like kinase 1 (Plk1).
商品
Quantitative and qualitative western blotting to validate knockdown by esiRNA.
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