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Merck
CN

P5998

Monoclonal Anti-hPlk1 (Polo Like Kinase 1) antibody produced in mouse

~2 mg/mL, clone 35-206, purified immunoglobulin, buffered aqueous solution

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UNSPSC Code:
12352203
eCl@ss:
32160410
NACRES:
NA.44
MDL number:
MFCD07370940

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产品名称

Monoclonal Anti-hPlk1 (Polo Like Kinase 1) antibody produced in mouse, ~2 mg/mL, clone 35-206, purified immunoglobulin, buffered aqueous solution

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

35-206, monoclonal

form

buffered aqueous solution

species reactivity

human, rat, mouse

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.04-0.08 μg/mL using whole extract of HeLa cell line

isotype

IgG2b

UniProt accession no.

P53350

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... PLK1(5347)
mouse ... Plk1(18817)
rat ... Plk1(25515)

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Application

Monoclonal Anti-hPlk1 (Polo Like Kinase 1) antibody produced in mouse has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry
  • immunofluorescence staining

Biochem/physiol Actions

PLK1 is a serine-threonine kinase that is expressed in mitotic cells and cancers. This kinase regulates mitotic entry by modulating cdc2/cyclin B and is also involved in Nlp-mediated nucleation of microtubules .
Polo Like Kinase 1 (Plk1) has a role in tumor progression. Plk1 is a target of the DNA damage checkpoint on the basis that its activity is inhibited by DNA damage in G2 and mitosis. Moreover, Plk1 was found to bind to the sequence-specific DNA binding domain of p53. This interaction between p53 and Plk1 inhibits the transcriptional activation and the proapoptotic functions of p53.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti- hPlk1 (Polo Like Kinase 1) (mouse IgG2b isotype) is derived from the 35-206 hybridoma produced by the fusion of mouse myeloma cells (PAI) and splenocytes from BALB/c mice immunized with human full length Plk1. Polo-like kinases (Plks) are a family of serine/threonine kinases. The Plk family is structurally and functionally related to the polo gene in Drosophila. Plk1 localizes during mitosis in centrosomes, kinetochores and in the central spindle.

Immunogen

human full length Plk1.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
059M4750V
033M4792V
035K4853

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Polo-like kinase 1 (Plk1) inhibits p53 function by physical interaction and phosphorylation
Ando K, et al.
The Journal of biological chemistry, 279(24), 25549-25561 (2004)
Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis
Neef R, et al.
The Journal of cell biology, 162(5), 863-876 (2003)
Association of spindle midzone particles with polo-like kinase 1 during meiosis in mouse and human oocytes
Otsuki J, et al.
Reproductive Biomedicine Online, 18(4), 522-528 (2009)
The crystal structure of the human polo-like kinase-1 polo box domain and its phospho-peptide complex
Cheng KY, et al.
The Embo Journal, 22(21), 5757-5768 (2003)
Identification of a novel Polo-like kinase 1 inhibitor that specifically blocks the functions of Polo-Box domain
Chen Y, et al.
Oncotarget, 8(1), 1234-1246 (2017)
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