form
liquid
analyte chemical class(es)
proteins (Immunoglobulins of various mammalian species)
technique(s)
affinity chromatography: suitable
immunoprecipitation (IP): suitable
capacity
~4 mg binding capacity (rabbit IgG)
shipped in
wet ice
storage temp.
−20°C
Quality Level
Application
EZview™ Red Protein A Affinity亲和凝胶已被用于纯化免疫沉淀和和染色质免疫沉淀（ChIP） 检测中的蛋白质。
当进行小规模的亲和力捕获（例如免疫沉淀）时，亲和基质很难在微量离心管中看到。 意外吸入树脂会导致结果的定量变化。 EZview™红色亲和凝胶大大降低了沉淀损失的风险。 EZview树脂的性能与传统的非彩色亲和凝胶一样，但使得用户可以轻松区分沉淀物与上清液。这与更准确的数据相关，因为损失的蛋白质更少。
Disclaimer
对于美国客户：本品含汞。废弃后切勿放入垃圾桶，须按照地方、州或联邦法律处置。
Features and Benefits
- 可见性增加-红色可降低意外吸入的风险
- 通过减少意外损失提高目标蛋白的回收率
- 重现性更高-收率更一致
General description
EZview™ Red Protein A亲和凝胶是一种易于观察的红色蛋白A亲和凝胶。亲和树脂含有通过共价键连接到交联4％琼脂糖珠上的金黄色葡萄球菌蛋白A。该亲和凝胶用于将抗体IgG 的FC部分与样品结合，如细胞裂解物和组织。结合EZview™ Red Protein A亲和凝胶的免疫复合物通过离心回收。红色染料增强了可视性，以获得更有效的结果。
Physical form
PBS中50%悬浮液加入50%甘油及0.0015%
含有50%甘油和15 ppm Kathon的PBS的1:1 (v/v)悬液。
Preparation Note
EZview™ Red Protein A亲和凝胶应当在-20 °C下在50%甘油中储存。按规定储存时本品可以在至少一年内保持稳定。 不可在没有甘油的情况下冷冻。
Legal Information
EZview is a trademark of Sigma-Aldrich Co. LLC
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
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相关内容
EZviewTM Red Protein A and ANTI-FLAG® M2 Affinity Gels: Immunoprecipitation with Enhanced Visibility Affinity Beads - Technical Article - July 2001
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