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Merck
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P6556

蛋白酶 K 来源于林伯氏白色念球菌

lyophilized powder, ≥30 units/mg protein

别名:

肽链内切酶

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关于此项目

化学文摘社编号:
39450-01-6
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
254-457-8
NACRES:
NA.54
MDL number:
MFCD00132129
EC 号:
3.4.21.64 (BRENDA, IUBMB)

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产品名称

蛋白酶 K 来源于林伯氏白色念球菌, lyophilized powder, ≥30 units/mg protein

form

lyophilized powder

specific activity

≥30 units/mg protein

mol wt

28.93 kDa

technique(s)

DNA extraction: suitable

solubility

H2O: soluble 1 mg/mL, clear, colorless

foreign activity

Dnase ≤30 Kunitz units/mg solid
RNase ≤0.003 Kunitz units/mg solid

shipped in

wet ice

storage temp.

−20°C

Quality Level

300

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Application

P6556产品为冻干粉形式。P6556产品已可用于分解人晶状体蛋白。蛋白酶K消化的蛋白酶印迹可以揭示蛋白质与蛋白质表面的相互作用。Sigma的该酶已用于鸡胚的预杂交。它还被用来富集PrPSc，一种存在于羊、仓鼠和老鼠痒病样本中的朊病毒蛋白。
在分离DNA和RNA的过程中，对核酸酶的蛋白水解失活起作用。
在分离DNA和RNA的过程中，用于核酸酶的蛋白水解失活。
用于去除内毒素结合的阳离子蛋白，如溶菌酶和核糖核酸酶A。
据报道，其可用于分离肝、酵母和绿豆线粒体
用于测定酶在细胞膜上的定位
用于石蜡包埋组织切片的处理，暴露用于抗体标记的抗原结合位点。
用于可传播海绵状脑病(TSE)研究中朊病毒脑组织样本蛋白的消化。
在分离DNA和RNA的过程中，蛋白酶K对核酸酶的蛋白水解失活起作用。
它被用于去除内毒素结合的阳离子蛋白，如溶菌酶和核糖核酸酶A。
它可用于分离肝、酵母和绿豆线粒体
，并用于测定酶在细胞膜上的定位
用于石蜡包埋组织切片的处理，暴露用于抗体标记的抗原结合位点，并
用于可传播海绵状脑病(TSE)研究中朊病毒脑组织样本蛋白的消化。 P6556产品为冻干粉形式。 P6556产品已可用于分解人晶状体蛋白。

Biochem/physiol Actions

蛋白酶K具有广泛的特异性，即使在天然状态下也能降解许多蛋白质。它主要裂解脂肪族和芳香族氨基酸羧基附近的含有封闭α-氨基的肽键。最佳pH值在7.5-9.0之间，等电点为8.9，其活性激活需要 Ca2+ (1-5 mM)。蛋白酶K可被二异丙基氟磷酸(DFIP)和苯甲磺酰氟(PMSF)抑制。
蛋白酶K是一种稳定且具有高活性的丝氨酸蛋白酶。晶体和分子结构研究证据表明该酶属于枯草素家族，具有活性位点催化三联体(Asp39-His69-Ser224)。它在一系列广泛的环境中均稳定：pH，缓冲盐，去污剂（SDS）以及温度。 在0.1-0.5% SDS存在的情况下，蛋白酶K可保持活性，在不损害分离DNA完整性的前提下，可以消化DNA制剂中的多种蛋白质和核酸酶。

Other Notes

一单位在37°C、pH 7.5条件下每分钟可水解尿素变性血红蛋白而产生相当于1.0μmole(181 μg)的酪氨酸显色（福林酚试剂显色）。

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

常规特殊物品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000505931
0000505931
0000486035
0000486035
0000486034

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Rongzhu Cheng et al.
The Journal of biological chemistry, 279(44), 45441-45449 (2004-08-19)
We report here the isolation of a novel acid-labile yellow chromophore from the enzymatic digest of human lens proteins and the identification of its chemical structure by liquid chromatography-mass spectrometry, liquid chromatography-tandem mass spectrometry, and (1)H, (13)C, and two-dimensional NMR.
M M Kristjánsson et al.
European journal of biochemistry, 260(3), 752-760 (1999-04-02)
An extracellular serine proteinase purified from cultures of a psychrotrophic Vibrio species (strain PA-44) belongs to the proteinase K family of the superfamily of subtilisin-like proteinases. The enzyme is secreted as a 47-kDa protein, but under mild heat treatment (30
Nathaniel Denkers et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 229(3), 651-657 (2004-03-03)
Multi-color whole-mount in situ hybridization is a powerful technique for comparing the spatial expression patterns of two or more genes in developing embryos. We have developed an amplified triple-label whole-mount fluorescence in situ hybridization (FISH) protocol that permits detection of
H Hilz et al.
European journal of biochemistry, 56(1), 103-108 (1975-08-01)
Hydrolysis of serum albumin by proteinase K was strongly (greater than 7-fold) stimulated by urea and dodecylsulfate in a dose-dependent manner. With an oligopeptide as substrate, however, proteinase K was inactivated by dodecylsulfate. This indicates that the apparent activation of
Michela Candelma et al.
Reproduction (Cambridge, England), 153(2), 123-132 (2016-11-03)
In vertebrates, the regulation of gametogenesis is under the control of gonadotropins (Gth), follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh). In fish, the physiological role of Gths is not fully understood, especially in species with asynchronous ovarian development. To elucidate
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实验方案

Proteinase K Enzymatic Assay

Proteinase K activity measured via spectrophotometry using hemoglobin substrate, crucial for enzyme characterization.

相关内容

Data Sheet - P6556

Instructions

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