产品名称
磷脂酶 C 来源于蜡样芽胞杆菌, ≥200 units/mg protein
form
lyophilized powder
specific activity
≥200 units/mg protein
mol wt
23-27 kDa by SDS-PAGE
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
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sustainability
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solubility
H2O: soluble 1.0 mg/mL, faintly hazy to hazy
greener alternative category
storage temp.
−20°C
Quality Level
Gene Information
Bacillus cereus ATCC 10987 ... plC(2749087)
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相关类别
Application
来自Sigma的酶已用于消化曼氏血吸虫成虫的磷脂酰丝氨酸部分。
磷脂酶C(PLC)可用于研究肾上腺素能受体介导的跨膜信号传导。它可用于降解含肌醇的磷脂。来自芽孢杆菌的磷脂酶C,产品P6621,已用于研究flhF是如何影响PLC和其他分泌性毒力因子的输出。
Biochem/physiol Actions
水解磷脂酰胆碱和其他甘油磷脂的磷酸键生成甘油二酯;该酶也可水解鞘磷脂、心磷脂、胆碱缩醛磷脂以及神经酰胺磷脂的磷酸键。
磷脂酶C可由凝血酶和血小板活化因子诱导形成1,2-二酰基甘油和磷脂酸。PLC可水解磷脂酰胆碱和其他甘油磷脂上的磷酸键,产生二酰基甘油;这种酶还可水解鞘磷脂、心磷脂、胆碱缩醛磷脂和神经酰胺磷脂的磷酸键。
General description
我们致力于为您带来更加绿色的替代产品,这些产品遵守一项或多项绿色化学12项原则。当用于生物柴油研究时,该产品的能效和废物预防能力已被增强。有关详细信息,请参阅 《Biofiles》的文章。
Other Notes
在pH 7.3、37 °C条件下,一单位在每分钟内可从L-α-磷脂酰胆碱释放1.0 μ摩尔的水溶性有机磷。
Physical form
含有约10%蛋白的冻干粉末。剩余;海藻糖、硫酸锌和磷酸钾
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
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T Das et al.
The Journal of biological chemistry, 268(36), 27398-27405 (1993-12-25)
Our studies on the alpha 1-adrenoceptor signaling have demonstrated that the Gh family is a signal mediator. We report here that a 69-kDa phospholipase C (PLC) is the effector in this signal pathway. The enzyme was isolated by dissociating a
Desiree van der Kleij et al.
The Journal of biological chemistry, 277(50), 48122-48129 (2002-10-03)
Schistosome infections are characterized by prominent T cell hyporesponsiveness during the chronic stage of infection. We found that schistosome-specific phosphatidylserine (PS) activated TLR2 and affected dendritic cells such that mature dendritic cells gained the ability to induce the development of
W Siess et al.
The Journal of biological chemistry, 259(13), 8286-8292 (1984-07-10)
The present study compares the molecular mechanism by which thrombin, platelet-activating factor, and epinephrine induce platelet activation. Thrombin and platelet-activating factor induce an initial activation of phospholipase C, as measured by formation of 1,2-diacylglycerol and phosphatidic acid, during platelet shape
Sara Salvetti et al.
Microbiology (Reading, England), 153(Pt 8), 2541-2552 (2007-07-31)
Flagellar arrangement is a highly conserved feature within bacterial species. However, only a few genes regulating cell flagellation have been described in polar flagellate bacteria. This report demonstrates that the arrangement of flagella in the peritrichous flagellate Bacillus cereus is
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