P7032
丙酮酸脱氢酶 来源于猪心脏
buffered aqueous glycerol solution, 0.1-1.0 units/mg protein (Lowry)
别名:
PDH, Pig heart pyruvate dehydrogenase
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Specific activity:
0.1-1.0 units/mg protein (Lowry)
Concentration:
≥5.0 mg/mL
form
buffered aqueous glycerol solution
Quality Level
specific activity
0.1-1.0 units/mg protein (Lowry)
concentration
≥5.0 mg/mL
impurities
polyethylene glycol, trace
foreign activity
α-ketoglutarate dehydrogenase ≤15%
shipped in
dry ice
storage temp.
−20°C
Application
丙酮酸脱氢酶有助于将糖酵解代谢途径与柠檬酸循环进行连接，并通过NADH释放能量。这种酶用于研究有氧训练如何影响丙酮酸脱氢酶，还用于研究丙酮酸脱氢酶激酶和丙酮酸脱氢酶复合物之间的相互作用，以及丙酮酸脱氢酶复合物的功能和调节。P7032产品以缓冲的甘油水溶液形式提供，来源于猪心脏。
该酶已用于初级酶测定以测试某些化合物还原PDK-（丙酮酸脱氢酶激酶）催化的PDC（丙酮酸脱氢酶复合物）失活的能力。
Biochem/physiol Actions
丙酮酸脱氢酶可参与到丙酮酸脱羧酶复合物（PDC）中丙酮酸的脱羧和硫辛酸的还原乙酰化。丙酮酸脱氢酶激酶（PDK）可使丙酮酸脱氢酶失活，而丙酮酸脱氢酶磷酸酶则能激活丙酮酸脱氢酶。
Physical form
以 50% 甘油溶液的形式提供，含有 ~9mg/mL 牛血清白蛋白、30% 蔗糖、1.5mM EDTA、1.5mM EGTA、1.5mM 2-巯基乙醇、0.3 TRITON® X-100、0.003% 叠氮化钠和 15mM 磷酸钾，pH 值为 6.8
Other Notes
在30℃、pH 7.4、饱和水平的辅酶A存在的条件下，一单位每分钟可将1.0 μm摩尔的β-NAD转化成β-NADH。
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - ED ENV 1
法规信息
低风险生物材料
此项目有
Function and regulation of mammalian pyruvate dehydrogenase complex. Acetylation, interlipoyl acetyl transfer, and migration of the pyruvate dehydrogenase component.
R L Cate et al.
The Journal of biological chemistry, 254(5), 1659-1665 (1979-03-10)
Insulin stimulation of pyruvate dehydrogenase in an isolated plasma membrane-mitochondrial mixture occurs by activation of pyruvate dehydrogenase phosphatase.
D A Popp et al.
The Journal of biological chemistry, 255(16), 7540-7543 (1980-08-25)
T D Aicher et al.
Bioorganic & medicinal chemistry letters, 9(15), 2223-2228 (1999-08-28)
Several oximes of triterpenes with a 17-beta hydroxyl and abietane derivatives are inhibitors of pyruvate dehydrogenase kinase (PDK) activity. The oxime 12 and dehydroabietyl amine 2 exhibit a blood glucose lowering effect in the diabetic ob/ob mouse after a single
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P7032-25UN | 04061832731261 |
| P7032-10UN | 04061832731254 |