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P7059

Sigma-Aldrich

磷酸盐缓冲溶液

10× PBS for Western blots and IP

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别名:
Phosphate buffered saline, PBS
MDL编号:
MFCD00131855
eCl@ss:
32129211
NACRES:
NA.25

等级

for molecular biology

质量水平

200

无菌性

0.2 μm filtered

形式

liquid

技术

immunoprecipitation (IP): suitable
western blot: suitable

pH值（酸碱度）

7.2-7.6(1x solution)

储存温度

room temp

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相关类别

一般描述

磷酸盐缓冲盐水（PBS）通常用作蛋白质印迹和免疫沉淀（IP）程序中的洗涤缓冲液，以及用于一般分子生物学应用的细胞裂解物的样品制备。

应用

适用于蛋白质印迹和IP的洗涤缓冲液，以及细胞裂解液样品制备。

组分

10x PBS 含有：
  • 30mM 磷酸钠
  • 1.5M 氯化钠
  • 10.5mM 磷酸钾

制备说明

使用分子生物级水将 10x PBS 稀释至 1x工作溶液。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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Sahar M Greish et al.
International journal of physiology, pathophysiology and pharmacology, 12(5), 134-146 (2020-11-24)
Hypertension can be induced by inhibiting nitric oxide synthesis with L-NAME, which also has a role in oxidative stress. Curcumin has strong antioxidant property. Our aim was to examine the possible preventive role of curcumin on renal dysfunction secondary to
Jennifer L S Sporty et al.
Analytical chemistry, 82(15), 6593-6600 (2010-07-14)
A novel method for extracting butyrylcholinesterase (BuChE) from serum as a means of identifying and measuring nerve agent adducts to human BuChE is presented here. Antibutyrylcholinesterase monoclonal antibodies were conjugated to protein-G ferromagnetic particles and mixed with 500 microL serum
Katie Burbridge et al.
Development (Cambridge, England), 148(8) (2021-04-30)
Body tissues are frequently exposed to stress, from toxic byproducts generated during cellular metabolism through to infection or wounding. Although it is well-established that tissues respond to exogenous injury by rapidly upregulating cytoprotective machinery, how energetically demanding tissues - vulnerable
Johan E Karlsson et al.
Drug metabolism and disposition: the biological fate of chemicals, 38(4), 705-714 (2010-01-15)
Membrane-bound transporter proteins play an important role in the efflux of drugs from cells and can significantly influence the pharmacokinetics of drug molecules. This study describes the production of large amounts of high-activity transporter membrane vesicles from human embryonic kidney
Shahani Noor et al.
Brain, behavior, and immunity, 61, 80-95 (2016-12-25)
A growing body of evidence indicates that prenatal alcohol exposure (PAE) may predispose individuals to secondary medical disabilities later in life. Animal models of PAE reveal neuroimmune sequelae such as elevated brain astrocyte and microglial activation with corresponding region-specific changes
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