Application
该产品已用于植物组织中焦磷酸盐含量的测定。通过监测马铃薯块茎提取物中的NADH氧化，它也已用作焦磷酸盐含量的快速常规检查。
Biochem/physiol Actions
每消耗一摩尔焦磷酸盐，两摩尔NADH被氧化为NAD+。在340 nm处通过分光光度法监测反应。
该试剂可用于检测10-100 nmole的焦磷酸盐。
该试剂可用于检测10-100 nmole的焦磷酸盐。
焦磷酸盐检测试剂盒将焦磷酸盐的产生与NADH的消耗偶联在一起。
Packaging
包括技术公告。
Preparation Note
将一瓶焦磷酸盐试剂的内容物溶于4.0 mL水中。
Analysis Note
一种包含果糖-6-磷酸激酶（焦磷酸依赖性）、醛缩酶、磷酸丙糖异构酶和α-甘油磷酸脱氢酶的偶联酶系统。
Other Notes
组成浓度
咪唑 × HCl, pH 7.4 45 mM
枸橼酸盐 5 mM
EDTA 0.10 mM
Mg2+, Mn2+, Co2+ 2 mM, 0.2 mM, 0.02 mM
β-NADH 0.8 mM
F-6-P 12 mM
牛血清白蛋白 5 mg/mL
糖稳定剂 5 mg/mL
PPi-PFK 0.5 单位/mL
醛缩酶 7.5 单位/mL
GDH 5 单位/mL
TPI 50 单位/mL
咪唑 × HCl, pH 7.4 45 mM
枸橼酸盐 5 mM
EDTA 0.10 mM
Mg2+, Mn2+, Co2+ 2 mM, 0.2 mM, 0.02 mM
β-NADH 0.8 mM
F-6-P 12 mM
牛血清白蛋白 5 mg/mL
糖稳定剂 5 mg/mL
PPi-PFK 0.5 单位/mL
醛缩酶 7.5 单位/mL
GDH 5 单位/mL
TPI 50 单位/mL
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
A continuous spectrophotometric assay for argininosuccinate synthetase based on pyrophosphate formation.
W E O'Brien
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R Viola et al.
Plant physiology, 97(2), 638-643 (1991-10-01)
Pretreatment of discs excised from developing tubers of potato (Solanum tuberosum L.) with 10 millimolar sodium fluoride induced a transient increase in 3-phosphoglycerate content. This was followed by increases in triose-phosphate, fructose 1,6-bisphosphate and hexose-phosphate (glucose 6-phosphate + fructose 6-phosphate
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D A Smyth et al.
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