Protein A−Alkaline Phosphatase

Protein A is a 42 kD cell surface protein isolated from Staphylococcus aureus. It exists in secreted as well as membrane associated forms and has high binding affinity for immunoglobulins. So, this property is useful for the labeling and purification of antibodies. Protein A−Alkaline Phosphatase can be used in immunoblotting to analyse the IgG glycosylation. Protein A−Alkaline Phosphatase has specificity for most of the mammals except goat, sheep and rat.

Protein A−alkaline phosphatase may be used in ELISA for analysis of IgG glycosylation.

Solution at approx. 1 mg protein/ml in 50 mM Tris, pH 8.0, 1.0 mM magnesium chloride, 0.1 mM ZnCl₂, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide.

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