P7634
3-磷酸甘油酸磷酸激酶 来源于面包酵母（酿酒酵母）
ammonium sulfate suspension, ≥500 units/mg protein
别名:
PGK, 磷酸甘油酸激酶, 3-磷酸甘油酸激酶, ATP：3-磷酸-D-甘油 1-磷酸转移酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-636-1
NACRES:
NA.54
MDL number:
Specific activity:
≥500 units/mg protein
Biological source:
bakers yeast
Concentration:
1.0-10.0 mg/mL
产品名称
3-磷酸甘油酸磷酸激酶 来源于面包酵母（酿酒酵母）, ammonium sulfate suspension, ≥500 units/mg protein
biological source
bakers yeast
form
ammonium sulfate suspension
specific activity
≥500 units/mg protein
storage condition
(Tightly closed)
concentration
1.0-10.0 mg/mL
foreign activity
Glyceraldehyde-3-phosphate dehydrogenase ≤0.1%
shipped in
wet ice
storage temp.
2-8°C
Quality Level
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Analysis Note
通过三氯乙酸(TCA)-双缩脲法测定的蛋白质。
Application
来自面包酵母的′3-磷酸甘油酸磷酸激酶（酿酒酵母）已用于：
- 研究低分子量GTP结合蛋白和甘油醛-3-磷酸脱氢酶的抑制机制
- 偶联法测定纯化兔骨骼肌腺嘌呤二核苷酸（NAD+）-依赖性甘油醛-3-磷酸脱氢酶（GAPDH）的反向活性
- 测定甘油醛-3-磷酸脱氢酶
Biochem/physiol Actions
3-磷酸甘油酸磷酸激酶催化磷酸基团从1,3-二磷酸甘油酸到ADP的可逆转移，以生成ATP和3-磷酸甘油酸。 3-磷酸甘油酸磷酸激酶的活性对糖酵解和糖异生作用是必需的。磷酸甘油酸激酶（PGK）通过催化两个产生ATP反应之一的糖酵解途径中发挥着重要的作用。它将1,3-二磷酸甘油酸（1,3BPGA）转化为3-磷酸甘油酸（3PGA）。此外，它还通过催化对峙反应生成1,3BPGA和二磷酸腺苷（ADP）参与糖异生过程。由于其参与除能量代谢之外的许多过程，如发病机制、与核酸的相互作用、肿瘤发生和发展、细胞死亡及病毒复制等，PGK还被称为兼职蛋白质。
General description
研究领域：细胞信号传导
磷酸甘油酸激酶（PGK）是一种糖酵解酶，可从多种生物体中分离获得。这种典型的铰链弯曲单体酶在三域生物中都很保守。这种酶由单体折叠多肽链构成，可分成两个几乎相同的结构域，每个结构域由两个螺旋链（螺旋链7和14）连接并由一个深裂隙分离。这种排列使该酶具有独特的双叶结构。
磷酸甘油酸激酶（PGK）是一种糖酵解酶，可从多种生物体中分离获得。这种典型的铰链弯曲单体酶在三域生物中都很保守。这种酶由单体折叠多肽链构成，可分成两个几乎相同的结构域，每个结构域由两个螺旋链（螺旋链7和14）连接并由一个深裂隙分离。这种排列使该酶具有独特的双叶结构。
Other Notes
一个单元将在pH6.9,25℃下每分钟将1.0μmol的1,3-二磷酸甘油酸转化为3-磷酸甘油酸。
Physical form
3.0 M (NH4)2SO4和 0.04 M 焦磷酸四钠溶液 (pH 8.0) 中的晶体混悬液
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Tobias Vöpel et al.
PloS one, 7(6), e39418-e39418 (2012-07-05)
The cytosol of a cell is a concentrated milieu of a variety of different molecules, including small molecules (salts and metabolites) and macromolecules such as nucleic acids, polysaccharides, proteins and large macromolecular complexes. Macromolecular crowding in the cytosolic environment is
Tyler J Boone et al.
The Journal of biological chemistry, 287(34), 29035-29044 (2012-07-05)
Phosphoglycerate kinase (PGK), present on the surface of group B streptococcus (GBS), has previously been demonstrated to bind the host proteins actin and plasminogen. The actin and plasminogen binding sites of GBS-PGK were identified using truncated GBS-PGK molecules, followed by
R A Wolosiuk et al.
The Journal of biological chemistry, 251(20), 6456-6461 (1976-10-25)
Chloroplast NADP-linked glyceraldehyde-3-phosphate dehydrogenase was resolved into three forms that differed in molecular weight: (a) larger than or equal to 1.5 million; (b) 600,000; and (c) less than or equal to 100,000. After preincubation with an effector (ATP, NADPH, or
I Pérez-Dorado et al.
Molecular oral microbiology, 27(4), 221-245 (2012-07-05)
Surface-exposed proteins of pathogenic bacteria are considered as potential virulence factors through their direct contribution to host-pathogen interactions. Four families of surface proteins decorate the cell surface of the human pathogen Streptococcus pneumoniae. Besides lipoproteins and LPXTG proteins, also present
Katarzyna A Broniowska et al.
American journal of physiology. Heart and circulatory physiology, 299(4), H1212-H1219 (2010-08-03)
S-nitrosothiols are nitric oxide (NO)-derived molecules found in biological systems. They have been variously discussed as both NO reservoirs and as major actors in NO-dependent, but cGMP-independent, signal transduction. Although S-nitrosation of specific cysteine residues has been suggested to represent
商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
相关内容
QC Methods
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