P7777
聚（乙二醇）
Hybri-Max™, mol wt 1,300-1,600, waxy solid, BioReagent, suitable for hybridoma
别名:
PEG
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关于此项目
线性分子式:
H(OCH2CH2)nOH
化学文摘社编号:
NACRES:
NA.75
PubChem Substance ID:
UNSPSC Code:
12352104
MDL number:
Technique(s):
cell culture | hybridoma: suitable
InChI
1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2
InChI key
LYCAIKOWRPUZTN-UHFFFAOYSA-N
biological source
synthetic (organic)
grade
Hybri-Max™
sterility
autoclaved
product line
BioReagent
form
waxy solid
mol wt
1,300-1,600
technique(s)
cell culture | hybridoma: suitable
impurities
endotoxin, tested
viscosity
25-35 cP (210 °F)
mp
40-50 °C
solubility
water: soluble (5 mL water/vial at 45 °C)
Quality Level
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Application
推荐用于细胞融合。
Preparation Note
在 45°C 或更高温度的水浴中熔化 PEG。使用无菌、预热的无血清培养基稀释至 30-50%（例如，对于 50% 溶液，向 5 gPEG 小瓶中加入 5 mL）。溶液的 pH 值应为微碱性（粉色，而不是橙色或紫色）。可能需要用无菌 NaOH 调节 pH 值。将溶液储存于 0°C 以下。不推荐长期保存或反复冻融。
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
281.5 °F - closed cup
flash_point_c
138.6 °C - closed cup
ppe
Eyeshields, Gloves, type N95 (US)
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