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Merck
CN

P8248

Sigma-Aldrich

Monoclonal Anti-Profilin antibody produced in mouse

~2 mg/mL, clone 2B8 (MAbPRF45a), purified immunoglobulin, buffered aqueous solution

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MDL编号:
UNSPSC代码:
12352203
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生物来源

mouse

偶联物

unconjugated

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

2B8 (MAbPRF45a), monoclonal

表单

buffered aqueous solution

分子量

antigen ~14 kDa

种属反应性

Arabidopsis thaliana

浓度

~2 mg/mL

技术

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 1-2 μg/mL using total extracts of Arabidopsis flower

同位素/亚型

IgG1

运输

dry ice

储存温度

−20°C

基因信息

Arabidopsis thaliana ... PRF4(829055)

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一般描述

Profilins are highly conserved pan-allergens that occur in plant foods, pollen and latex. Profilins have five distinct isoforms (PRF1-5) that bind with actin filaments to regulate cytoskeletal organization. PRF4 and PRF5 are cytoplasmic proteins that are expressed in mature pollens . Monoclonal Anti-Profilin antibody is specific for Arabidopsis pollen profilin (PRF4 and PRF5). The antibody detects tobacco and maize profilin (approx. 14 kDa), but does not react with constitutive specific profilins (PRF1, 2, and 3).

免疫原

profilin PRF4 protein.

应用

Monoclonal Anti-Profilin antibody is suitable for use in western blot (1-2 μg/mL using total extracts of Arabidopsis flower), indirect ELISA and immunohistochemistry.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品
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分析证书(COA)

Lot/Batch Number

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Muthugapatti K Kandasamy et al.
Cell motility and the cytoskeleton, 52(1), 22-32 (2002-04-27)
Profilin is a low-molecular weight, actin monomer-binding protein that regulates the organization of actin cytoskeleton in eukaryotes, including higher plants. Unlike the simple human or yeast systems, the model plant Arabidopsis has an ancient and highly divergent multi-gene family encoding
Alexandra Santos et al.
International archives of allergy and immunology, 155(3), 191-204 (2011-02-05)
Profilins are ubiquitous proteins, present in all eukaryotic cells and identified as allergens in pollen, latex and plant foods. The highly conserved structure justifies the cross-reactive nature of IgE antibodies against plant profilins and their designation as pan-allergens. Primary sensitization

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