General description
蛋白A是金黄色葡萄球菌的细胞壁成分，分子量为56kDa。它具有五个结合结构域，可与Fc或Fab片段的免疫球蛋白重链发生相互作用。蛋白A可激活补体系统、干扰素产生、超敏反应和细胞介导的细胞毒性作用。辣根过氧化物酶是一种含血红素的酶。 在过氧化氢存在下，该酶催化鲁米诺的氧化。
Application
来自金黄色葡萄球菌/辣根的蛋白A-过氧化物酶已用于酶联免疫吸附测定（ELISA）。
Biochem/physiol Actions
仅与大多数哺乳动物的IgG结合，除了大鼠、山羊、绵羊。
Packaging
包装规格基于蛋白含量。
Physical form
冻干粉末，含有柠檬酸钠缓冲盐。通过A205测定蛋白质。
Preparation Note
通过改良O′Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978)中所报道的有利于低分子量偶联物的实验方法，使用VI型过氧化物酶标记细胞外蛋白A。
Disclaimer
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
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实验方案
This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.
To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.
Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).
该实验程序用于使用连苯三酚作为底物测定过氧化物酶的酶活性。
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P8651-1MG | 04061826739778 |
| P8651-.5MG | 04061834401612 |
| P8651-2MG | 04061834401629 |
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