P8787
邻苯二胺 二盐酸盐
chromogenic, tablet
别名:
1,2-Phenylenediamine tablet, OPD, OPD Tablet
产品名称
邻苯二胺 二盐酸盐, tablet, 4 mg substrate per tablet
质量水平
表单
tablet
mp
258 °C (dec.) (lit.)
储存温度
2-8°C
SMILES字符串
Cl[H].Cl[H].Nc1ccccc1N
InChI
1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
InChI key
RIIWUGSYXOBDMC-UHFFFAOYSA-N
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一般描述
邻苯二胺二盐酸盐（OPD）是一种显色底物，因为利用辣根过氧化物酶缀合物，因此可用于酶联免疫吸附试验（ELISA）程序。底物产生可溶的最终产物，其颜色为橙棕色，并且可以在450 m下通过分光光度计读取。可以用3N HCl或3M H2SO4终止OPD反应，并在492 nm处读取。
应用
在酶联免疫吸附试验（ELISA）中，邻苯二胺盐酸盐用作辣根过氧化物酶偶联二抗的底物。
制备说明
将 1 片片剂溶于 pH 值为 5.0的0.05M磷酸盐 - 柠檬酸盐缓冲液中至所需浓度（通常使用0.4mg / ml的OPD浓度）。临用前，向 100 mL 底物缓冲液中加入 40μl 新鲜过氧化氢（浓度为 30%）。片剂单独包装在铝箔包装中。
警示用语：
Warning
危险分类
Aquatic Chronic 2 - Carc. 2 - Muta. 2 - Skin Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Kaustuv Nayak et al.
Virology, 558, 13-21 (2021-03-12)
India is one of the most affected countries by COVID-19 pandemic; but little is understood regarding immune responses to SARS-CoV-2 in this region. Herein we examined SARS-CoV-2 neutralizing antibodies, IgG, IgM, IgA and memory B cells in COVID-19 recovered individual
Min Wen Ku et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 28(8), 1772-1782 (2020-06-03)
Zika virus, a member of the Flaviviridae family, is primarily transmitted by infected Aedes species mosquitoes. In 2016, Zika infection emerged as a global health emergency for its explosive spread and the remarkable neurological defects in the developing fetus. Development
Esther Steiner et al.
BMC veterinary research, 5, 45-45 (2009-12-24)
Porcine circovirus type 2 (PCV2) is a dominant causative agent of postweaning multisystemic wasting syndrome (PMWS), a multifactorial disease complex with putative immunosuppressive characteristics. Little is known about adaptive PCV2-specific immune responses in infected pigs. Therefore, the T and B
Oluwaseyi Adekunle et al.
PLoS neglected tropical diseases, 15(9), e0009743-e0009743 (2021-09-04)
Vibrio cholerae is a bacterial pathogen which causes the severe acute diarrheal disease cholera. Given that a symptomatic incident of cholera can lead to long term protection, a thorough understanding of the immune response to this pathogen is needed to
Aaron J Schmitz et al.
Immunity, 54(9), 2159-2166 (2021-09-01)
The emergence of SARS-CoV-2 antigenic variants with increased transmissibility is a public health threat. Some variants show substantial resistance to neutralization by SARS-CoV-2 infection- or vaccination-induced antibodies. Here, we analyzed receptor binding domain-binding monoclonal antibodies derived from SARS-CoV-2 mRNA vaccine-elicited
商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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