P8804
磷脂酶 C,磷脂酰肌醇特异性 来源于蜡样芽胞杆菌
buffered aqueous glycerol solution, ≥1,000 units/mg protein (Lowry)
别名:
1-磷脂酰- D - 肌醇肌醇磷酸水解酶,环磷酸盐形成, PI-PLC
生物来源
Bacillus sp. (Bacillus cereus)
质量水平
表单
buffered aqueous glycerol solution
比活
≥1,000 units/mg protein (Lowry)
分子量
28 kDa
异质活性
Phopholipase C (lecithinase) ≤1 units/mg protein
Sphingomyelinase ≤40 units/mg protein
储存温度
2-8°C
基因信息
Bacillus cereus E33L ... BCZK3513(3026815)
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一般描述
来自蜡状芽孢杆菌的磷脂酶 C(PI-PLC)具有不规则的三糖磷酸异构酶(TIM)-桶结构,具有八个标准的平行 β 桶。它是一种 28 kDa 的蛋白质。
应用
蜡状芽孢杆菌特有的磷脂酶 C(磷脂酰肌醇)可用于:
- 裂解底物对硝基苯基磷酰胆碱(p-NPPC)和对硝基苯基磷酰磷酸(p-NPP)
- 裂解 lynx1 蛋白的糖基磷脂酰肌醇(GPI)锚定及其与质膜的分离
- 裂解免疫标记的 HeLa 细胞
生化/生理作用
用于释放锚定的 GPI 膜中的蛋白质。
磷脂酶 C(PI-PLC)通过裂解糖基磷脂酰肌醇释放二酰基甘油。它具有广泛的底物特异性,其活性受金属离子和表面活性剂的影响。
外形
含 10 mM Tris-HCl,pH 8.0 和 10 mM EDTA 的 60%(v/v)甘油溶液
分析说明
根据 Ellman 等人 的研究测定乙酰胆碱酯酶。
其他说明
在 30°C、pH 7.4 条件下,每分钟释放 1 个单位的乙酰胆碱酯酶。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Lynx1 shifts alpha4beta2 nicotinic receptor subunit stoichiometry by affecting assembly in the endoplasmic reticulum
Nichols WA, et al.
The Journal of Biological Chemistry, 289(45), 31423-31432 (2014)
High-level expression of recombinant phospholipase C from Bacillus cereus in Pichia pastoris and its characterization
Seo KH and Rhee JI
Biotechnology Letters, 26(19), 1475-1479 (2004)
Ligand binding characteristics of a glycosylphosphatidyl inositol membrane-anchored HeLa cell folate receptor epitope-related to human milk folate binding protein
Holm J, et al.
Bioscience Reports, 20(2), 109-118 (2000)
Crystal structure of phosphatidylinositol-specific phospholipase C from Bacillus cereus in complex with glucosaminyl (alpha1? 6)-d-myo-inositol, an essential fragment of GPI anchors
Heinz DW, et al.
Biochemistry, 35(29), 9496-9504 (1996)
Keisuke Matsuda et al.
Endocrinology, 156(3), 934-946 (2014-12-17)
Adiponectin (Adipo), a multimeric adipocyte-secreted protein abundant in the circulation, is implicated in cardiovascular protective functions. Recent work documented that Adipo locally associates with responsive tissues through interactions with T-cadherin (Tcad), an atypical, glycosylphosphatidylinositol (GPI)-anchored cadherin cell surface glycoprotein. Mice
商品
GPI锚定糖蛋白
Glycosylphosphatidylinisotol- (GPI) anchored proteins have been identified throughout a broad range of eukaryotic species ranging from humans to insects, yeasts, bacteria, and fungi, suggesting that they are an ancient modification.
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