P8811
蛋白酶 来源于灰色链霉菌
powder, BioReagent, suitable for mouse embryo cell culture, ≥3.5 units/mg solid
别名:
Actinase E, Pronase E
登录 查看组织和合同定价。
选择尺寸
关于此项目
化学文摘社编号:
UNSPSC Code:
12352207
EC Number:
232-909-5
NACRES:
NA.75
eCl@ss:
32160410
MDL number:
产品名称
蛋白酶 来源于灰色链霉菌, powder, BioReagent, suitable for mouse embryo cell culture, ≥3.5 units/mg solid
product line
BioReagent
form
powder
specific activity
≥3.5 units/mg solid
technique(s)
cell culture | embryo: suitable
impurities
starch, essentially free
storage temp.
−20°C
Quality Level
正在寻找类似产品? 访问 产品对比指南
Analysis Note
含有约 25％ 的醋酸钙。
Application
蛋白酶通常用于核酸的分离过程，通过补充0.2％十二烷基硫酸钠和10 mM EDTA并孵育0.5-3.0小时。
该酶比已知碱性蛋白酶在更高的pH范围内更具活性，即使在0.2N NaOH溶液中也显示出了蛋白水解活性。该酶用于不溶性蛋白的蛋白水解和蛋白的结构研究。
Biochem/physiol Actions
一种具有包括细胞外丝氨酸蛋白酶在内至少三种蛋白水解活性的混合物。通常，丝氨酸蛋白酶可显示出广泛的底物特异性，而这被视为通过分子中由一个Asp、一个His和Ser残基组成的活性位点所介导的。该酶更倾向于水解谷氨酸或天冬氨酸的羧基侧的肽键。
General description
通过在80 °C以上加热15-20分钟以完全灭活。
Other Notes
一种不常见的非特异性蛋白酶。
在 pH7.5，37℃ 下，一个单元每分钟将水解酪蛋白，并产生相当于 1.0 μmole（181 μg）酪氨酸的颜色（使用 Folin-Ciocalteu 试剂显色）。
Preparation Note
收集自灰色链霉菌的培养基肉汤中。
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
S Blumberg et al.
European journal of biochemistry, 136(1), 151-154 (1983-10-17)
A series of 2-mercaptoacetyl-dipeptides, a potential group of metalloendopeptidase inhibitors, has been synthesized by coupling the N-hydroxysuccinimide ester of S-acetyl-2-mercaptoacetic acid with hydrophobic dipeptide methyl ester hydrochlorides, followed by hydrolysis with NaOH in aqueous methanol and acidification with HCl. Thus
Shantha K Mahadevaiah et al.
Nature, 586(7830), 612-617 (2020-08-21)
Single-cell RNA sequencing of embryos can resolve the transcriptional landscape of development at unprecedented resolution. To date, single-cell RNA-sequencing studies of mammalian embryos have focused exclusively on eutherian species. Analysis of mammalian outgroups has the potential to identify deeply conserved
J Erik Mylroie et al.
Environmental toxicology and chemistry, 40(3), 780-791 (2020-10-13)
Perfluorooctanesulfonic acid (PFOS) is a perfluorinated compound used in many industrial and consumer products. It has been linked to a broad range of adverse effects in several species, including zebrafish (Danio rerio). The zebrafish embryo is a widely used vertebrate
Xuan Mu et al.
Macromolecular bioscience, 20(1), e1900191-e1900191 (2019-08-23)
Hierarchical molecular assembly is a fundamental strategy for manufacturing protein structures in nature. However, to translate this natural strategy into advanced digital manufacturing like three-dimensional (3D) printing remains a technical challenge. This work presents a 3D printing technique with silk
Archana Vijaya Kumar et al.
Cancer science, 111(8), 2907-2922 (2020-06-24)
Heparan sulfate proteoglycans (HSPGs) act as signaling co-receptors by interaction of their sulfated glycosaminoglycan chains with numerous signaling molecules. In breast cancer, the function of heparan sulfate 2-O-sulfotransferase (HS2ST1), the enzyme mediating 2-O-sulfation of HS, is largely unknown. Hence, a
商品
Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture
用于转基因小鼠胚胎培养的小鼠胚胎培养基及经过胚胎培养验证的试剂
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持