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经验公式(希尔记法):
C17H19ClN2O
化学文摘社编号:
分子量:
302.80
EC Number:
202-147-8
UNSPSC Code:
12171500
NACRES:
NA.52
PubChem Substance ID:
MDL number:
Colour Index Number:
45005
Beilstein/REAXYS Number:
3795789
产品名称
派洛宁Y, for NA electrophoresis
InChI key
INCIMLINXXICKS-UHFFFAOYSA-M
InChI
1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1
SMILES string
[Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C
grade
Molecular Biology
form
powder
composition
Dye content, ≥45%
technique(s)
nucleic acid detection: suitable
mp
250-260 °C (lit.)
solubility
water: 10 mg/mL, dark red
suitability
suitable for nucleic acid staining
storage temp.
room temp
Quality Level
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Application
派洛宁Y已被用于:
- 休眠和循环造血干细胞的纯化和骨髓细胞(HSC)的染色
- 作为多氯联苯溶液 (PCBS) 的成分用于乳腺侧群细胞 (MG-SP) 的染色
- CD34+细胞的染色
Biochem/physiol Actions
派洛宁Y是一种插入RNA 的阳离子染料,在电泳中显示为红色条带。研究表明,它还可以在活细胞的线粒体中聚集。派洛宁Y还可以与甲基绿或Nancy-520一起使用,用作石蜡组织切片中核酸的荧光差异染料。
Preparation Note
该产品可溶于水(1 mg/ml),产生澄清的深红色溶液。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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A gel electrophoresis method for determining the relative binding constants of biologically active, intercalating fluorescent stains.
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Analytical biochemistry, 95(2), 329-339 (1979-06-01)
Photosensitizing effects of the tricyclic heteroaromatic cationic dyes pyronin Y and toluidine blue O (tolonium chloride
Darzynkiewicz Z and Carter SP
Cancer research, 48(5) (1988)
Staining of RNA after polyacrylamide gel electrophoresis.
K Marcinka
Methods in cell biology, 10, 49-68 (1975-01-01)
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