生物来源
microbial (T.album
T. ALBUM)
质量水平
表单
lyophilized powder
标记范围
30-140 units per g solid
技术
DNA extraction: suitable
RNA extraction: suitable
protein extraction: suitable
溶胀
1 g swells to ~5 mL
储存温度
−20°C
应用
蛋白酶K-琼脂糖被用于分离蛋白质和核酸、修饰酶和固定酶。蛋白酶K-琼脂糖已被用于研究家犬的肺炎克雷伯菌（肺炎克雷伯菌）和多房棘球绦虫（Echinococcus multilocularis）感染。
生化/生理作用
通过将蛋白酶K固定在不溶性基质上，如琼脂糖，可将其用于更多应用中。琼脂糖在大多数系统中都是惰性的，并且每单位重量具有很高的活性。利用这种反应性树脂，通过酶与底物在合适介质中的瞬时接触，能够迅速催化酶反应。反应可以在间歇桨液反应器中进行，甚至可以在小柱中进行。无论是哪种情况，不溶性酶都不会污染反应混合物，可以很容易地将其从间歇桨液中滤出或留在色谱柱中。
特点和优势
•基质载体上的可回收蛋白酶 K。
•可以利用旋转柱处理或分批处理。
•可以利用旋转柱处理或分批处理。
外形
用乳糖稳定化的冻干粉
其他说明
在pH 7.5、37℃条件下，一单位每分钟可水解尿素变性血红蛋白并产生相当于1.0 μ摩尔 (181 μg) 酪氨酸的显色（使用Folin-Ciocalteu试剂显色）。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
David da Silva Barreira et al.
Scientific reports, 13(1), 1163-1163 (2023-01-21)
Biofilms represent a major concern in the food industry and healthcare. The use of probiotic bacteria and their derivatives as an alternative to conventional treatments to fight biofilm development is a promising option that has provided convincing results in the
Zohar Erez et al.
Nature, 541(7638), 488-493 (2017-01-19)
Temperate viruses can become dormant in their host cells, a process called lysogeny. In every infection, such viruses decide between the lytic and the lysogenic cycles, that is, whether to replicate and lyse their host or to lysogenize and keep
Hirokazu Kouguchi et al.
Experimental parasitology, 128(1), 50-56 (2011-02-09)
We show that a conventionally purified glycoprotein component of Echinococcus multilocularis protoscolex, designated as Emgp-89, may be useful as a serodiagnostic antigen for detecting E. multilocularis infection in dogs domesticated in endemic areas. Emgp-89 was obtained from the parasite material
Yi Zuo et al.
PloS one, 14(10), e0223299-e0223299 (2019-10-04)
Oral herpes is a highly prevalent infection caused by herpes simplex virus 1 (HSV-1). After an initial infection of the oral cavity, HSV-1 remains latent in sensory neurons of the trigeminal ganglia. Episodic reactivation of the virus leads to the
M J Pearse et al.
Immunology letters, 18(3), 219-223 (1988-07-01)
This report describes a method for preparing both DNA and RNA simultaneously from as few as 5 X 10(5) lymphoid cells. The method is suitable for cultured cells or any tissue from which a cell suspension can be prepared and
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