P9620

嘌呤霉素

Name: 嘌呤霉素
Brand: SIGMA

Ready Made Solution, from Streptomyces alboniger, 10 mg/mL in H₂O, suitable for cell culture

别名:

3′-(L-α-Amino-p-methoxyhydrocinnamamido)-3′-deoxy-N,N-dimethyladenosine dihydrochloride, Stylomycin dihydrochloride

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关于此项目

化学文摘社编号:

58-58-2

NACRES:

NA.85

PubChem Substance ID:

329820352

UNSPSC Code:

51281906

MDL number:

MFCD00012691

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产品名称

嘌呤霉素, Ready Made Solution, from Streptomyces alboniger, 10 mg/mL in H₂O, suitable for cell culture

InChI key

MKSVFGKWZLUTTO-FZFAUISWSA-N

SMILES string

Cl.Cl.COc1ccc(C[C@H](N)C(=O)N[C@H]2[C@@H](O)[C@@H](O[C@@H]2CO)n3cnc4c(ncnc34)N(C)C)cc1

InChI

1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16+,18+,22+;;/m0../s1

biological source

Streptomyces alboniger

type

for cell culture

assay

>98% (HPLC)

form

ready-to-use solution

storage condition

(Store in cool place. Keep container tightly closed in a dry and well -ventilated place. )

concentration

10 mg/mL in H₂O

technique(s)

cell culture | mammalian: suitable

color

colorless to yellow

solubility

H₂O: soluble 10 mg/mL

antibiotic activity spectrum

Gram-positive bacteria
neoplastics
parasites

mode of action

protein synthesis | interferes

shipped in

wet ice

storage temp.

−20°C

Quality Level

300

Application

嘌呤霉素是一种氨基核苷酸抗生素，衍生自白黑链霉菌。它可以选择包含抗性基因嘌呤霉素 N-乙酰基转移酶（PAC）的细胞。它被用在兔模型中，研究处理后的血管平滑肌细胞的生存能力。嘌呤霉素用在体细胞克隆和胚胎移植后产生增强的绿色荧光蛋白（EGFP）转基因仔猪。

Biochem/physiol Actions

作用机制：嘌呤霉素通过提前终止链来抑制蛋白质合成，作为氨基酰基-tRNA 的 3′ 末端的类似物起作用。嘌呤霉素还可作为二肽基肽酶 II（丝氨酸肽酶）和胞质溶胶丙氨酰氨基肽酶的可逆抑制剂起作用。

耐药性机制：嘌呤霉素乙酰转移酶是有效的抗性基因。

抗菌谱：该产品对革兰氏阳性微生物有活性，对耐酸杆菌的活性较低，对革兰氏阴性微生物的活性更低。嘌呤霉素可以防止细菌、原生动物、藻类和哺乳动物细胞的生长，并且起效迅速，可在 2 天内杀死 99％的细胞。

Features and Benefits

适用于多种研究应用的优质抗生素
抗生素和蛋白质合成抑制剂
有效抑制原核和真核细胞
通过干扰RNA功能抑制蛋白质合成
常用作细胞生物学研究中的选择剂

General description

嘌呤霉素，也称杆菌肽或嘌啉霉素盐酸盐，是一种氨基核酸酶抗生素和蛋白质合成抑制剂。它是由土壤放线菌白黑链霉菌（Streptomyces alboniger）通过一系列酶促反应合成。嘌呤霉素有效抑制原核和真核细胞，其作用原理是干扰RNA功能，从而抑制蛋白质合成。它也充当氨基肽酶和脑啡肽酶的抑制剂。嘌呤霉素可以使细菌、原生动物、水藻和哺乳动物细胞的生长迅速停止，但是含pac基因的细胞有耐药性。 pac基因的基因产物是嘌呤霉素-N-乙酰基转移酶，这种酶通过乙酰化反应使嘌呤霉素失活。

嘌呤霉素与tRNA的含氨基末端结构相似，这一特性令其在蛋白质合成过程中进入核糖体，结合新生多肽链，并使链的延长停止。嘌呤霉素广泛用于细胞培养，可作为以耐嘌呤霉素基因转化的细胞的选择剂。

Other Notes

保存于密闭容器内，置于干燥通风处。

如需了解生化试剂系列的更多信息，请填写此表。

Preparation Note

将该产物以 10 mg/mL 的浓度浓缩在水中。已在转染了 pac 耐药基因的 HeLa 细胞上测试了其生长抑制和细胞选择的能力。

存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

涉药品监管产品

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

0000484599

0000404679

0000404678

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