跳转至内容
Merck
CN
登录查看公司和协议定价

About This Item

UNSPSC代码:
41105904
NACRES:
NE.02

包装

pkg of 5 vials (5x200µL aliquots )

浓度

≥5x108 VP/ml (via p24 Assay)

应用

CRISPR

运输

dry ice

储存温度

−70°C

一般描述

The human, paired guide Poison (pgPoison) library targets 3` splice sites with paired guide RNAs for alternative exon removal (pgRNAs), induce skipping of "poison" cassette exons and corresponding upstream constitutive exons in ultraconserved regions. This compact library (targets 963 exons) allows direct comparison between loss of a constitutive coding exon (knockout) with loss of a poison exon.

Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.

应用

  • Functional Genomics/Target Validation
  • Dual-gRNA
  • Poison exon
  • Internal controls included

特点和优势

  • Focus on your research, and we will generate your lentivirus screening library
  • Use CRISPR nucleases to knockout protein-coding genes to assess their function.
  • Built-in enrichment and depletion controls allow researchers to gauge the success of their pooled screening experiments confidently.
  • Dual-Guide Vector System (pools are gRNA-only, Cas9 sold separately) See products: LVCAS9BST or LVCAS9NEO.
  • Ease of optimization: Utilizes BFP and Puromycin as selection markers under EF1alpha promoter.

原理

In traditional CRISPR KO screens, Cas9 introduces double-strand breaks at locations specified by a gRNA. In this library, a CRISPR-Cas9-based method is used by expressing two sgRNAs to manipulate isoforms independent of gene inactivation. This approach enables rapid suppression of exon recognition in polyclonal settings to identify functional roles for individual exons.

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 文件 section.

如需帮助,请联系 客户支持

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持