应用
检测限：最大50ng胰蛋白酶
蛋白酶荧光检测试剂盒已用于：
- 通过基于酪蛋白的蛋白酶测定，检测蛋白水解活性
- 检测真菌菌株的细胞外蛋白水解活性
- 定量线粒体蛋白酶活性
- 测定土壤溶液中的蛋白酶生成
制备说明
提供了足以用于200次一毫升反应体系的试剂。
三氯乙酸（TCA）组分以6.1 N TCA 溶液的形式提供。0.6 N TC A溶液可通过将所需体积的 T0699 用超纯水进行10倍稀释而制备。使用试剂盒时无需将所有体积的 T0699 均进行稀释。
三氯乙酸（TCA）组分以6.1 N TCA 溶液的形式提供。0.6 N TC A溶液可通过将所需体积的 T0699 用超纯水进行10倍稀释而制备。使用试剂盒时无需将所有体积的 T0699 均进行稀释。
分析说明
已测试过其在全部4种蛋白酶水解类别中的检测效果（丝氨酸、天冬氨酸、半胱氨酸和金属蛋白酶）。
仅试剂盒组分
产品编号
说明
- Incubation buffer 25 mL
- FITC-casein solution 5 mL
- Assay buffer 200 mL
- Protease standard (trypsin) 20 μg
- FITC control 50 mg
- Trichloroacetic acid (TCA) solution 30 mL
警示用语：
Danger
危险分类
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3
靶器官
Respiratory system
储存分类代码
8A - Combustible corrosive hazardous materials
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
低风险生物材料
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商品
Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.
实验方案
Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.
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