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Merck
CN

PKH67GL

PKH67 Green Fluorescent Cell Linker Kit,用于常规细胞膜标记

Distributed for Phanos Technologies

别名:

Green PKH membrane labeling kit

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关于此项目

NACRES:
NA.32
UNSPSC Code:
12352207
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Quality Level

packaging

pkg of 1 kit

storage condition

protect from light

fluorescence

λex 490 nm; λem 502 nm (PKH67 dye)

detection method

fluorometric

shipped in

ambient

storage temp.

room temp

Application

用于常规细胞膜标记的PKH26红色荧光细胞连接子试剂盒已用于:
  • 标记并进一步研究在三阴性乳腺癌状况下从细胞中排出的外泌体。
  • 标记凋亡性细胞,用于研究ανβ5受体在凋亡性细胞的结合和内化中的作用。
  • 在荧光成像中。

本试剂盒适用于常规细胞膜标记。PKH67具有长于PKH1和PKH2的脂肪炭尾,后两者是另外两种之前常用于体外体内细胞示踪的绿色染料。由于炭尾长度更长,内部研究已经证明PKH67比PKH2由更少的细胞间转移。
在采用PKH1和PKH2进行的体内研究中,荧光强度都会缓慢损失。由于这是绿色细胞linker染料而非红色细胞linker染料出现的行为特征,因而PKH67会出现类似的性质。不分裂细胞的体外细胞膜留存和体内荧光半衰期的关联性揭示,PKH67的体内荧光半衰期为10-12天。其他具有类似半衰期的绿色细胞linker染料已经被用于监测1-2月内的体内 淋巴细胞和巨噬细胞运输,结果表明PKH67还可用于中等时长的体内跟踪研究。

Other Notes

如需关于PKH及CellVue®的荧光细胞连接子染料的更多技术详情,包括大量的参考书目,请访问这里

Legal Information

CellVue is a registered trademark of Phanos Technologies

仅试剂盒组分

产品编号
说明

  • Diluent C 6 x 10

  • PKH67 Cell Linker in ethanol .5 mL

pictograms

FlameExclamation mark

signalword

Danger

hcodes

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

存储类别

3 - Flammable liquids

flash_point_f

57.2 °F - closed cup

flash_point_c

14 °C - closed cup

法规信息

危险化学品

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历史批次信息供参考:

分析证书(COA)

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Anjali P Kusumbe et al.
Stem cells (Dayton, Ohio), 27(3), 498-508 (2009-03-04)
Recruitment and localization of endothelial precursors within tumors is a potential area for the development of therapeutics, because their functional contribution to tumor vasculature is realized to be important for cancer cell survival. However, the exact nature of the recruited
Exosomes from triple-negative breast cancer cells can transfer phenotypic traits representing their cells of origin to secondary cells
O Brien K, et al.
European Journal of Cancer, 49(8), 1845-1859 (2013)
Hybrid microscaffold-based 3D bioprinting of multi-cellular constructs with high compressive strength: A new biofabrication strategy
Yu Jun T, et al.
Scientific Reports, 6, 39140-39140 (2016)
Dandan Ma et al.
Cells, 9(4) (2020-04-01)
Human salivary histatin 1 (Hst1) and Hst2 exhibit a series of cell-activating properties (e.g., promoting adhesion, spreading, migration and metabolic activity of mammalian cells). In contrast, Hst5 shows an anti-fungal property but no cell-activating properties. Previous findings suggest that their
alpha v beta 5 integrin recruits the CrkII-Dock180-Rac1 complex for phagocytosis of apoptotic cells
Albert, Matthew L and Kim, Jong-Ii and Birge, Raymond B
Nature Cell Biology, 2(12), 899-899 (2000)

商品

Lipophilic cell tracking dyes enable cancer biologists to track tumor and immune cell functions both in vitro and in vivo. Read the article to choose a right membrane dye kit for cell tracking and proliferation monitoring.

Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.

PKH and CellVue® Fluorescent Cell Linker Kits provide fluorescent labeling of live cells over an extended period of time, with no apparent toxic effects.

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

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全球贸易项目编号

货号GTIN
PKH67GL-1KT04061836680947

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