用途
sufficient for 15 purifications
质量水平
环保替代产品特性
Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
技术
DNA purification: suitable
环保替代产品分类
, Aligned
储存温度
15-25°C
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一般描述
GenElute无内毒素质粒制备试剂盒可以简单、快速、低成本的纯化质粒DNA（内毒素水平≤0.1 EU/μg），所得产物可用于高效转染。 内毒素（也称为脂多糖或LPS）常常会与质粒DNA共纯化，这会显著降低内毒素敏感细胞系的转染效率。
取过夜培养物离心收集重组大肠杆菌，采用改良的SDS碱裂解法处理得到澄清的裂解液。通过简单的萃取相分离步骤去除内毒素。在高盐环境下，质粒DNA吸附到硅胶膜上接受进一步的纯化。简单的离心洗涤后，用无内毒素的水洗脱吸附的质粒DNA。25-200 mL培养物可以制备出多达1.2 mg不含内毒素的质粒DNA。
回收的质粒DNA主要以超螺旋形式存在。此类DNA可直接用于转染、限制性酶切、克隆、测序和PCR扩增等下游应用。
取过夜培养物离心收集重组大肠杆菌，采用改良的SDS碱裂解法处理得到澄清的裂解液。通过简单的萃取相分离步骤去除内毒素。在高盐环境下，质粒DNA吸附到硅胶膜上接受进一步的纯化。简单的离心洗涤后，用无内毒素的水洗脱吸附的质粒DNA。25-200 mL培养物可以制备出多达1.2 mg不含内毒素的质粒DNA。
回收的质粒DNA主要以超螺旋形式存在。此类DNA可直接用于转染、限制性酶切、克隆、测序和PCR扩增等下游应用。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。相比使用苯酚和氯仿的标准DNA提取方法，该产品是一种更安全的化学产品。
应用
该试剂盒已用于纯化质粒脱氧核糖核酸，用于高效转染。
特点和优势
- 转染效率高，产量高
- 纯化至 250μg 无内毒素质粒 DNA（≤0.1 EU/μg DNA）
- 快速简单，允许在 2 小时内完成 12 次制备
包装
<strong>Components included:</strong><UL><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>E4274<strong> Component description: </strong>Endotoxin Removal solution</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>W4011<strong> Component description: </strong>Optional Wash Solution</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>N2409<strong> Component description: </strong>Neutralization Solution</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>G6665<strong> Component description: </strong>GenElute™ Maxiprep Binding Columns in tubes</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>C2112<strong> Component description: </strong>Column Preparation Solution</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>B1555<strong> Component description: </strong>DNA Binding Solution</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>R1149<strong> Component description: </strong>Resuspension Solution</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>2107<strong> Component description: </strong>Endotoxin-Free Water</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>L1912<strong> Component description: </strong>Lysis Solution</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>W3886<strong> Component description: </strong>Wash Solution Concentrate</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>R6148<strong> Component description: </strong>RNase A solution</LI><LI><strong>Qty in kit:</strong>1<strong> Part number: </strong>C4353<strong> Component description: </strong>Collection Tubes, 50mL capacity</LI></UL>
法律信息
GenElute is a trademark of Sigma-Aldrich Co. LLC
警示用语：
Danger
危险分类
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Central nervous system
储存分类代码
8A - Combustible corrosive hazardous materials
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
危险化学品
低风险生物材料
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
ErbB4 expression in neural progenitor cells (ST14A) is necessary to mediate neuregulin-1beta1-induced migration.
Gambarotta G
The Journal of Biological Chemistry, 279(47), 48808-48816 (2004)
Registered report: Senescence surveillance of pre-malignant hepatocytes limits liver cancer development
Samrrah Raouf
eLife, 4, e04105-e04105 (2015)
Registered report: Melanoma genome sequencing reveals frequent PREX2 mutations
Denise Chroscinski
eLife, 3, e04180-e04180 (2014)
Registered report: tumour vascularization via endothelial differentiation of glioblastoma stem-like cells
Denise Chroscinski
eLife, 4, e04363-e04363 (2015)
Ming-Wei Chen et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(36), 13538-13543 (2008-09-04)
H5N1 influenza viruses have spread extensively among wild birds and domestic poultry. Cross-species transmission of these viruses to humans has been documented in over 380 cases, with a mortality rate of approximately 60%. There is great concern that a H5N1
相关内容
Tools for plasmid preparation, in vitro transcription, mRNA purification, and LNP formulation in mRNA-based vaccine and therapeutic development.
用于质粒制备、体外转录、mRNA 纯化和 LNP 制剂的工具，用于基于 mRNA 的疫苗和疗法开发。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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