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Merck
CN

PLN70

GenElute™质粒小量制备试剂盒

greener alternative

sufficient for 70 purifications

别名:

GenElute™质粒试剂盒, Gen Elute

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关于此项目

NACRES:
NA.52
UNSPSC Code:
41105501

主要文件

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产品名称

GenElute™质粒小量制备试剂盒, sufficient for 70 purifications

usage

sufficient for 70 purifications

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

DNA purification: suitable

greener alternative category

, Aligned

storage temp.

15-25°C

Quality Level

200

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相关类别

Application

此类DNA可直接用于限制性酶切、克隆、PCR、转化、转录、传统和自动化测序等下游应用。

Features and Benefits

  • 可在30分钟内获得纯化的质粒DNA
  • 至多可纯化出15 μg质粒DNA
  • 检测不到基因组DNA或RNA污染
  • 无需进行苯酚/氯仿萃取或乙醇沉淀步骤

General description

GenElute 质粒小量制备试剂盒可以简单、快速、低成本的从重组大肠杆菌培养物中分离质粒DNA。该试剂盒结合了硅胶结合技术和离心柱形式的便利性，可在30分钟内从1-5 mL大肠杆菌培养物中回收出至多15 μg高拷贝数质粒DNA。请注意，实际得率和最佳的培养物体积取决于质粒和培养基。

通过离心分离法收集过夜培养的重组大肠杆菌培养物，并通过改性SDS碱裂解法进行处理，之后再在高盐环境下使DNA吸附到硅胶膜上。随后通过离心洗涤步骤去除污染物。最后，在水或Tris-EDTA缓冲液中洗脱结合所得的DNA。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。相比使用苯酚和氯仿的标准DNA提取方法，该产品是一种更安全的化学产品。
Click here to view the kit protocol

Other Notes

更多信息，请见www.sigma-aldrich.com/genelutehp。

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Central nervous system

存储类别

3 - Flammable liquids

法规信息

危险化学品
低风险生物材料
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000431800
0000431800
0000434404
0000434404
SLBL5378V

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实验方案

Escort™ IV Transfection Reagent Protocol

Product manual provides detailed protocol for easy DNA transfection.

Escort™ III Transfection Reagent Protocol

The product bulletin providin detailed use protocol for easy DNA transfection.

相关内容

mRNA Synthesis

Tools for plasmid preparation, in vitro transcription, mRNA purification, and LNP formulation in mRNA-based vaccine and therapeutic development.

mRNA 合成

用于质粒制备、体外转录、mRNA 纯化和 LNP 制剂的工具，用于基于 mRNA 的疫苗和疗法开发。

GenElute Plasmid Miniprep Kit
User Guide - PLN70,PLN350
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