产品名称
GenElute™ 质粒大量制备试剂盒, sufficient for 15 purifications
usage
sufficient for 15 purifications
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
DNA purification: suitable
greener alternative category
storage temp.
15-25°C
Quality Level
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Application
回收的质粒DNA主要以超螺旋形式存在。用琼脂糖凝胶电泳没有检测出可见的基因组DNA或RNA污染。回收的DNA可直接用于下游应用使用，如限制性酶切，连接，测序，PCR和转染。
General description
GenElute 质粒大量制备试剂盒为简单、快速、低成本的从大肠杆菌培养物中分离质粒DNA。该试剂盒结合了硅胶结合技术和离心柱形式的便利性，可在45分钟内从25-200 ml LB培养基中回收出多达1.2mg的质粒DNA。请注意，实际得率和最佳的培养物体积取决于质粒和培养基。
通过离心分离法收集过夜培养的重组大肠杆菌培养物，并通过改性SDS碱裂解法进行处理，之后再在高盐环境下使DNA吸附到硅胶膜上。随后通过离心洗涤步骤去除污染物。最后，在水或Tris-EDTA缓冲液中洗脱结合所得的DNA。
Sigma Life Science致力于为您提供符合一项或多项绿色化学十二条原则 （The 12 Principles of Greener Chemistry），更环保的替代产品。相对于使用苯酚和氯仿进行DNA提取的标准方法，本产品符合固有安全化学原则(Inherently Safer Chemistry)。
通过离心分离法收集过夜培养的重组大肠杆菌培养物，并通过改性SDS碱裂解法进行处理，之后再在高盐环境下使DNA吸附到硅胶膜上。随后通过离心洗涤步骤去除污染物。最后，在水或Tris-EDTA缓冲液中洗脱结合所得的DNA。
Sigma Life Science致力于为您提供符合一项或多项绿色化学十二条原则 （The 12 Principles of Greener Chemistry），更环保的替代产品。相对于使用苯酚和氯仿进行DNA提取的标准方法，本产品符合固有安全化学原则(Inherently Safer Chemistry)。
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Central nervous system
存储类别
8A - Combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Han Liu et al.
FEBS letters, 583(14), 2365-2371 (2009-06-24)
The chaperonin GroEL contains two seven-subunit rings, and allosteric signals between them are required to complete the GroEL reaction cycle. For this reason SR1, a mutant of GroEL that forms only single rings, cannot function as a chaperone. Mutations in
Characterisation of mutations in GroES that allow GroEL to function as a single ring.
Liu H
Febs Letters, 583(14), 2365-2371 (2009)
Martina Herrmann et al.
Applied and environmental microbiology, 75(10), 3127-3136 (2009-03-24)
Communities of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in freshwater sediments and those in association with the root system of the macrophyte species Littorella uniflora, Juncus bulbosus, and Myriophyllum alterniflorum were compared for seven oligotrophic to mesotrophic softwater lakes and
实验方案
Product manual provides detailed protocol for easy DNA transfection.
The product bulletin providin detailed use protocol for easy DNA transfection.
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