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Sigma-Aldrich

Trypsin Profile IGD Kit

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别名:
In gel digestion kit, Proteomics grade Trypsin, TPCK treated
NACRES:
NA.56

用途

 kit sufficient for ≤100 applications

质量水平

200

储存温度

2-8°C

相关类别

一般描述

Trypsin Profile IGD Kit 能够对多达 100 个切除的蛋白点进行快速、高效和完全的凝胶内胰蛋白酶消化。消化后的蛋白质可用于 MALDI-MS（基质辅助激光解吸/电离质谱法），无需额外制备。由于 Trypsin Profile IGD Kit 含有蛋白质组学级别的胰蛋白酶，在 MALDI 光谱中观察到较高的序列覆盖率和较少的模棱两可的自溶峰。
通过赖氨酸的ε-氨基的还原甲基化对胰蛋白酶进行化学修饰，减少自溶，使自溶片段最小化。此外，还经过 TPCK（对甲苯磺酰基苯丙氨酰基氯甲基酮）处理，去除残留的糜蛋白酶活性，然后通过亲和层析进一步纯化，得到了适合蛋白质组学工作的高纯度胰蛋白酶。

应用

胰蛋白酶谱 IGD 试剂盒已用于 LC-MS（液相色谱-质谱法）/MS 和 MALDI-TOF（基质辅助激光解吸电离-飞行时间）质谱分析的凝胶内消化。

特点和优势

  • 相比其它凝胶中消化试剂盒来说可更快速地完成脱色
  • 包含对感兴趣蛋白/多肽进行脱色、消化及提取所需的所有试剂
  • 兼容使用Coomassie, SYPRO® Orange, SYPRO Ruby, and ProteoSilver™等进行染色的凝胶
  • 所得样品可即用于MALDI-MS或HPLC-MS分析

法律信息

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC
SYPRO is a registered trademark of Life Technologies

仅试剂盒组分

产品编号
说明
  • Destaining Solution Reconstituted to 75

警示用语：

Danger

危险分类

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

3 - Flammable liquids

WGK

WGK 2

闪点(°F)

35.6 °F - closed cup

闪点(°C)

2.0 °C - closed cup

法规信息

危险化学品

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Identification of plant viruses using one-dimensional gel electrophoresis and peptide mass fingerprints.
Luo, H., et al.
J. Virol. Methods, 165(2), 297-301 (2010)
X-M Lu et al.
International journal of burns and trauma, 3(1), 37-48 (2013-02-07)
Insulin resistance is a major effect of burn injury and insulin receptor substrate-1 (IRS-1) plays an important role in signal transduction. Here, we explored the integrity of IRS-1 in muscle after burn injury. A murine model of severe burn injury
Shuo Han et al.
Cell death & disease, 12(11), 1070-1070 (2021-11-12)
Uncontrolled overactivation of autophagy may lead to autophagic cell death, suppression of which is a pro-survival strategy for tumors. However, mechanisms involving key regulators in modulating autophagic cell death remain poorly defined. Here, we report a novel long noncoding RNA
Xingyuan Liu et al.
Cell death & disease, 13(7), 642-642 (2022-07-24)
Hepatocellular carcinoma (HCC) is the most common subtype of liver cancer and the second most fatal cancer in the world despite the great therapeutic advances in the past two decades, which reminds us of the gap in fully understanding the
Irene Riz et al.
Methods in molecular biology (Clifton, N.J.), 699, 431-447 (2010-12-01)
In vivo biotinylation tagging, based on a method in which a protein of interest is tagged with a peptide that is biotinylated in vivo by coexpression of Escherichia coli BirA biotin ligase, has been successfully used for the isolation of
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