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QR0200

Sigma-Aldrich

定量 RT-PCR ReadyMix™

One step RT-qPCR for probe-based methods, MMLV & hot-start Taq

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别名:
1 步法 RT-qPCR 混合液, 实时定量 PCR 预混液
NACRES:
NA.55

质量水平

300

用途

sufficient for 250 reactions

特点

dNTPs included
hotstart

技术

RT-qPCR: suitable

颜色

colorless

输入

purified RNA

相容性

ABI 5700
 ABI 7000
 ABI 7300
 ABI 7500 Fast
 ABI 7500
 ABI 7700
 ABI 7900 HT
ABI 7900 HT Fast
 ABI 7900
 ABI StepOne
 ABI StepOnePlus
 ABI ViiA 7
 Bio-Rad CFX384
 Bio-Rad CFX96
 Bio-Rad MJ Chromo4
 Bio-Rad MJ Opticon 2
 Bio-Rad MJ Opticon
 Bio-Rad MiniOpticon
 Bio-Rad MyiQ
 Bio-Rad iCycler iQ
 Bio-Rad iQ5
 Cepheid SmartCycler
 Eppendorf® Mastercycler ep realplex2 s
 Eppendorf® Mastercycler ep realplex
 Illumina Eco qPCR
 Qiagen Corbett Rotor-Gene 3000
 Qiagen Corbett Rotor-Gene 6000
 Qiagen Corbett Rotor-Gene Q
 Roche LightCycler 480
 Strategene Mx3000P
 Strategene Mx3005P
 Strategene Mx4000

检测方法

probe-based

运输

wet ice

储存温度

−20°C

相关类别

一般描述

定量RT-PCR ReadyMix™是设计用于测量基因表达的一步式RT-PCR试剂盒，其结合了莫洛尼鼠白血病病毒逆转录酶（M-MLV RT）和JumpStart ™Taq DNA聚合酶。这种方便的2X预混液包括M-MLV RT、JumpStart ™Taq DNA聚合酶、纯度99％的脱氧核苷酸、缓冲液、玻璃钝化剂和稳定剂。JumpStart ™Taq DNA聚合酶是一种抗体灭活的热启动酶（hot-start enzyme）。一旦反应温度达到70°C，DNA聚合酶-抗体复合物随即解离，Taq DNA聚合酶活性得以恢复。这种抗体-酶复合物可实现简单便捷的设置，并相对于手动热启动技术具有更低的污染风险。该试剂盒经过设计可用于基于荧光杂交探针的检测方法。

应用

定量RT-PCR ReadyMix™已被用于通过定量逆转录聚合酶链反应（RT-qPCR）来定量测定特定表达基因的信使RNA（mRNA）水平。

特点和优势

  • 该主混合物（master mix）可确保不同反应之间的一致性和可再现性。
  • 降低了多个移液步骤的污染风险
  • 与手动或热启动（Hot Start）方法相比，减少了设置时间
  • JumpStart™Taq聚合酶可减少引物二聚体和非特异性产物的形成
  • 广泛的仪器兼容性
  • 包括一个单独的ROX参考染料瓶，用于反应标准化

包装

1个试剂盒足以用于每次20 μL的250次反应或每次50 μL的100次反应。

法律信息

本品仅供研究使用。
Eppendorf is a registered trademark of Eppendorf AG
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

仅试剂盒组分

产品编号
说明
  • Probe Based qRT-PCR ReadyMix 2 X
  • Moloney Murine Leukemia Viral Reverse Transcriptase (M-MLV RT) 5000 U

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • P219210X PCR Buffer, Optimized for routine PCR with MgCl2 included 1.5 mL/vial化学品安全说明书
  • M8787Magnesium chloride solution, PCR Reagent, 25 mM MgCI2 solution for PCR 25 mM化学品安全说明书
  • P219210X PCR Buffer, Optimized for routine PCR with MgCl2 included 100 X化学品安全说明书

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象形图

Exclamation markEnvironment
GHS07,GHS09

警示用语：

Warning

危险分类

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

含少量动物源组分生物产品
常规特殊物品

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Validation of Quantitative PCR Assays
Lovatt, A., et al.
BioPharm., 22-32 (2002)
An Integrated High-Throughput System for mRNA Purification and Quantitation for Use in Identifying Gene Knockdown by RNA Interference
Wang, et al.
JALA: Journal of the Association for Laboratory Automation, 11, 314-318 (2006)
A screen of shRNAs targeting tumor suppressor genes to identify factors involved in A549 paclitaxel sensitivity
Ji D, et al.
Oncology Reports, 18(6), 1499-1505 (2007)
T B Morrison et al.
BioTechniques, 24(6), 954-958 (1998-06-19)
Continuous fluorescence observation of amplifying DNA allows rapid and accurate quantification of initial transcript copy number. A simple and generic method for monitoring product synthesis with the double-stranded DNA dye, SYBR Green I provides initial template copy number estimation limited
S A Bustin
Journal of molecular endocrinology, 29(1), 23-39 (2002-08-30)
The fluorescence-based real-time reverse transcription PCR (RT-PCR) is widely used for the quantification of steady-state mRNA levels and is a critical tool for basic research, molecular medicine and biotechnology. Assays are easy to perform, capable of high throughput, and can

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实验方案

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Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

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