R0155
Anti-Anti-RanGAP1 (C-terminal) 兔抗
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
别名:
Anti-Ran-GTPase-Activating Protein 1
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~65 kDa
species reactivity
human
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~1 mg/mL
technique(s)
indirect immunofluorescence: 5-10 μg/mL using HeLa cells, indirect immunofluorescence: suitable, western blot: 1-2 μg/mL using HEK-293T cells transfected with human RanGAP1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... RANGAP1(5905)
mouse ... Rangap1(19387)
General description
The unmodified form of RanGAP1 (65 kDa) is exclusively cytoplasmic, whereas the 90 kDa SUMO-1-modified form is associated with the cytoplasmic fibers of the nuclear pore complex (NPC).
Immunogen
synthetic peptide corresponding to amino acids 573-587 located at the C-terminus of human RanGAP1. The sequence is highly conserved (80% identity) in mouse RanGAP1.
Application
Anti-RanGAP1 (C-terminal) antibody produced in rabbit has been used for immunoblotting and listeriolysin O (LLO) purification.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.
Biochem/physiol Actions
RanGAP1 (Ran GTPase Activating Protein 1, 65 kDa) is a key regulator of Ran activity, specifically inducing the GTPase activity of Ran. RanGAP1 is conjugated to the small ubiquitin-related modifier protein SUMO-1. The association of RanGAP1 with RanBP2 facilitates nuclear transport.
Physical form
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
Listeria monocytogenes impairs SUMOylation for efficient infection
Ribet D, et al.
Nature, 464(7292), 1192-1192 (2010)
The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo
Joseph J, et al.
Current Biology, 14(7), 611-617 (2004)
David Ribet et al.
Nature, 464(7292), 1192-1195 (2010-04-24)
During infection, pathogenic bacteria manipulate the host cell in various ways to allow their own replication, propagation and escape from host immune responses. Post-translational modifications are unique mechanisms that allow cells to rapidly, locally and specifically modify activity or interactions
Salmonella Engages Host MicroRNAs To Modulate SUMOylation: a New Arsenal for Intracellular Survival.
Smriti Verma et al.
Molecular and cellular biology, 35(17), 2932-2946 (2015-06-24)
Posttranslational modifications (PTMs) can alter many fundamental properties of a protein. One or combinations of them have been known to regulate the dynamics of many cellular pathways and consequently regulate all vital processes. Understandably, pathogens have evolved sophisticated strategies to
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