R0280
Anti-RanGAP1 兔抗
affinity isolated antibody, buffered aqueous solution, ~2 mg/mL
别名:
Anti-Ran-GTPase-Activating Protein 1
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~65 kDa
species reactivity
human
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~2 mg/mL
technique(s)
indirect immunofluorescence: 10-20 μg/mL using HeLa cells, western blot: 1-2 μg/mL using HEK-293T cells transfected with human RanGAP1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... RANGAP1(5905)
mouse ... Rangap1(19387)
相关类别
General description
Ran GTPase−activating protein 1 (RanGAP1), a guanosine-5′-triphosphatase (GTPase)-activating protein for Ran exists in two different types, where one is cytoplasmic and other is concerted at the cytoplasmic fibers of nuclear pore complexes (NPCs). Mammalian RanGAP1 has a C-terminal extension. Ran GTPase gene is located on human chromosome 22q13.2.
Immunogen
synthetic peptide corresponding to amino acids 1-20 located at the N-terminus of human RanGAP1, conjugated to KLH. This sequence is identical in mouse RanGAP1.
Application
Anti-RanGAP1 (N-terminal) antibody produced in rabbit may be used immunoblotting and immunofluorescence.
Biochem/physiol Actions
Anti-RanGAP1 (N-terminal) recognizes human RanGAP1.
Ran GTPase−activating protein 1 (RanGAP1) regulates Ran activity. RanGAP1 can stimulate the GTPase activity of Ran. Ran is also regulated by a chromatin-bound nucleotide exchange factor, regulator of chromosome condensation 1 (RCC1) that keeps Ran in the active guanosine-5′-triphosphate (GTP)-bound state. RanGAP1 has the ability to conjugate to the small ubiquitin-related modifier protein small ubiquitin-related modifier 1 (SUMO-1). RanGAP1 may participate in nuclear protein import.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
低风险生物材料
常规特殊物品
此项目有
Ubc9p and the conjugation of SUMO-1 to RanGAP1 and RanBP2
Saitoh H, et al.
Current Biology, 8(2), 121-124 (1998)
Circular RNA circ-RanGAP1 regulates VEGFA expression by targeting miR-877-3p to facilitate gastric cancer invasion and metastasis
Lu J, et al.
Cancer Letters, 471, 38-48 (2020)
SUMO-1 modification and its role in targeting the Ran GTPase-activating protein, RanGAP1, to the nuclear pore complex
Matunis MJ, et al.
The Journal of cell biology, 140(3), 499-509 (1998)
A small ubiquitin-related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2
Mahajan R, et al.
Cell, 88(1), 97-107 (1997)
RanGAP1 induces GTPase activity of nuclear Ras-related Ran
Bischoff FR, et al.
Proceedings of the National Academy of Sciences of the USA, 91(7), 2587-2591 (1994)
相关内容
Instructions
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