Monoclonal Anti-Rat IgG2a antibody produced in mouse

Immunoglobulins (IgGs) are glycoprotein antibodies that modulate several immune responses. Rat IgGs have four subclasses, namely, IgG1, IgG2a, IgG2b, and IgG2c.

Monoclonal Anti-Rat IgG2a (mouse IgG1 isotype) is derived from the R2A-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified rat IgG.

Purified rat IgG

Monoclonal Anti-Rat IgG2a antibody produced in mouse has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• slot blot immunoassay
• immunoblotting
• dot blot
• immunocytochemistry

Monoclonal Anti-Rat IgG2a recognizes an epitope located on the heavy chain of rat IgG2a. The antibody detects rat IgG2a derived from normal serum or myeloma proteins, but not the other rat immunoglobulins. Weak cross-reaction is observed with guinea pig immunoglobulins but not with IgG and serum preparations of bovine, cat, chicken, dog, goat, horse, human, mouse, pig, rabbit, or sheep.

Rat immunoglobulins (IgGs) against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgG is a useful tool for the analysis of target proteins. Monoclonal Anti-Rat IgG2a antibody is specific for epitope on the heavy chain of rat IgG2a. The antibody localizes the denatured-reduced heavy chain molecule of rat IgG2a (γ2a). The product recognizes rat IgG2a derived from normal serum or myeloma proteins, but does not bind to other rat immunoglobulins. The antibody weakly associates with guinea pig immunoglobulins but does not react with IgG and serum preparations derived from bovine, cat, chicken, dog, goat, horse, human, mouse, pig, rabbit, and sheep.

The product is provided as ascites fluid with 0.1% sodium azide as a preservative.

For continuous use, store at 0-5 °C. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use,

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.