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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-809-1
MDL number:
Specific activity:
300,000-600,000 units/mg protein
Biological source:
Aspergillus sp. (Aspergillus oryzae )
biological source
Aspergillus sp. (
Quality Segment
form
ammonium sulfate suspension
specific activity
300,000-600,000 units/mg protein
mol wt
11068 by amino acid sequence
technique(s)
cell based assay: suitable
input
general use sample(s)
suitability
suitable for separating native or denatured proteins, or nucleic acids
application(s)
cell analysis
storage temp.
2-8°C
Application
来自 米曲霉的核糖核酸酶T1(RNase T1)可用于在测序之前对变性的RNA进行消化并用于蛋白折叠的研究 。
Biochem/physiol Actions
来自米曲霉的核糖核酸酶T1(RNase T1)是一种内切核糖核酸酶,其可在G残基后进行水解。切割发生在胍核糖核苷酸的3'-磷酸基团和相邻核苷酸的5'-羟基之间。初始产物为2':3'环磷酸核苷,其可水解成相应的3'-核苷磷酸。它与胰腺RNase的不同之处在于它可特异性攻击鸟嘌呤位点以产生具有3′-GMP末端基团的3′-GMP和寡核苷酸。
Physical form
2.8 M (NH4)2SO4 溶液混悬液
Analysis Note
E1%/280法测定蛋白
Other Notes
在pH7,5,37°C条件下,一个单位可在15分钟内产生相当于 ΔA260 为 1.0 的酸可溶性寡核苷酸,反应体积为1.0 mL。底物:酵母RNA。
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves