R1003
核糖核酸酶T1 来源于米曲霉
ammonium sulfate suspension, 300,000-600,000 units/mg protein
别名:
核糖核苷-3′-脒基-寡核苷酸水解酶, 鸟粪核糖核酸酶
产品名称
核糖核酸酶T1 来源于米曲霉, ammonium sulfate suspension, 300,000-600,000 units/mg protein
biological source
Aspergillus sp. (Aspergillus oryzae)
form
ammonium sulfate suspension
specific activity
300,000-600,000 units/mg protein
mol wt
11068 by amino acid sequence
technique(s)
cell based assay: suitable
suitability
suitable for separating native or denatured proteins, or nucleic acids
application(s)
cell analysis
storage temp.
2-8°C
Quality Level
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Analysis Note
E1%/280法测定蛋白
Application
来自 米曲霉的核糖核酸酶T1（RNase T1）可用于在测序之前对变性的RNA进行消化并用于蛋白折叠的研究 。
Biochem/physiol Actions
来自米曲霉的核糖核酸酶T1（RNase T1）是一种内切核糖核酸酶，其可在G残基后进行水解。切割发生在胍核糖核苷酸的3'-磷酸基团和相邻核苷酸的5'-羟基之间。初始产物为2'：3'环磷酸核苷，其可水解成相应的3'-核苷磷酸。它与胰腺RNase的不同之处在于它可特异性攻击鸟嘌呤位点以产生具有3′-GMP末端基团的3′-GMP和寡核苷酸。
Other Notes
在pH7，5,37°C条件下，一个单位可在15分钟内产生相当于 ΔA260 为 1.0 的酸可溶性寡核苷酸，反应体积为1.0 mL。底物：酵母RNA。
Physical form
2.8 M (NH4)2SO4 溶液混悬液
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
法规信息
常规特殊物品
此项目有
C Nick Pace et al.
Journal of molecular biology, 408(3), 514-528 (2011-03-08)
Our goal was to gain a better understanding of the contribution of hydrophobic interactions to protein stability. We measured the change in conformational stability, Δ(ΔG), for hydrophobic mutants of four proteins: villin headpiece subdomain (VHP) with 36 residues, a surface
Scott Quarrier et al.
RNA (New York, N.Y.), 16(6), 1108-1117 (2010-04-24)
Structure mapping experiments (using probes such as dimethyl sulfate [DMS], kethoxal, and T1 and V1 RNases) are used to determine the secondary structures of RNA molecules. The process is iterative, combining the results of several probes with constrained minimum free-energy
Rasmus Lybech Jensen et al.
Journal of the American Chemical Society, 134(23), 9820-9826 (2012-05-19)
Singlet molecular oxygen, O(2)(a(1)Δ(g)), can influence many processes pertinent to the function of biological systems, including events that result in cell death. Many of these processes involve a reaction between singlet oxygen and a given amino acid in a protein.
S Dubey et al.
Journal of controlled release : official journal of the Controlled Release Society, 152(3), 356-362 (2011-03-15)
Cathodal iontophoresis of anionic macromolecules has been considered a major challenge owing to (i) the presence of a negative charge on the skin under physiological conditions and (ii) the electroosmotic solvent flow in the (opposite) anode-to-cathode direction. Moreover, electroosmosis, and
Isil Severcan et al.
Nature chemistry, 2(9), 772-779 (2010-08-24)
Supramolecular assembly is a powerful strategy used by nature to build nanoscale architectures with predefined sizes and shapes. With synthetic systems, however, numerous challenges remain to be solved before precise control over the synthesis, folding and assembly of rationally designed
商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
相关内容
Product Information Sheet
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