grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
≥10000 units/mL, 10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Application
HindIII, a restriction endonuclease, is used in molecular biology applications to cleave DNA at the recognition site 5′-A/AGCTT-3′ to generate DNA fragments with cohesive 5′-ends.
Biochem/physiol Actions
Recognition sequence: 5′-A/AGCTT-3′
Cutting results: 2-10-fold Hind III overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 65 °C for 15 minutes
Cutting results: 2-10-fold Hind III overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 65 °C for 15 minutes
Physical form
Solution in 10 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 1 mM dithioerythritol, 250 mM NaCl, 0.01% polydocanol (v/v), 50% glycerol (v/v) at 4 °C
Other Notes
Comment: Hind III under suboptimal reaction conditions will cleave secondary recognition sites (star activity).
Supplied with 10x Restriction Enzyme Buffer SB (B8781)
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Stephen J King et al.
Molecular biology of the cell, 14(12), 5089-5097 (2003-10-21)
Cytoplasmic dynein and dynactin are megadalton-sized multisubunit molecules that function together as a cytoskeletal motor. In the present study, we explore the mechanism of dynein-dynactin binding in vitro and then extend our findings to an in vivo context. Solution binding
Tomás Brdicka et al.
The Journal of experimental medicine, 196(12), 1617-1626 (2002-12-18)
A key molecule necessary for activation of T lymphocytes through their antigen-specific T cell receptor (TCR) is the transmembrane adaptor protein LAT (linker for activation of T cells). Upon TCR engagement, LAT becomes rapidly tyrosine phosphorylated and then serves as
M Hsu et al.
Biochemistry, 17(1), 131-138 (1978-01-10)
In the presence of 100 mM Tris buffer (pH 7.5) and 1-10 mM Mg2+ EcoRI endonuclease cleaves DNA at a specific nucleotide sequence and in a characteristic way: -GAATTC-. But if Mg2+ is replaced by Mn2+, the specificity of the
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Nico Mitro et al.
Methods in molecular biology (Clifton, N.J.), 952, 137-144 (2012-10-27)
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional approach, site-directed mutagenesis
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持