Merck
CN

R1780

Sigma-Aldrich

RPMI-1640 培养基

With L-glutamine and sodium bicarbonate. Without arginine, leucine, lysine, and phenol red, liquid, sterile-filtered, suitable for cell culture, designed for isotope labeling for cell culture applications

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MDL编号:
NACRES:
NA.75

质量水平

无菌性

sterile-filtered

形式

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

NaHCO3: 2 g/L
phenol red: no
glucose: 2 g/L (Dextro)
L-glutamine: 0.3 g/L

运输

ambient

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一般描述

RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成,用于在悬浮培养中支持淋巴母细胞生长,但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用,但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。

重悬

设计用于细胞培养应用中氨基酸稳定同位素标记。
补充 0.100 g/L L -精氨酸- 13 C 6 15 N 4 HCl(产品 608033)、0.100 g/L L -亮氨酸- 13 C 6 15 N(产品 608068)和 0.100 g/L L -赖氨酸- 13 C 6 15 N 2(产品 608041)。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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Mengying Ke et al.
Frontiers in oncology, 11, 779562-779562 (2021-11-23)
Multiple myeloma (MM) is a hematological malignancy worldwide in urgent need for novel therapeutic strategies. Since Velcade (bortezomib) was approved for the treatment of relapsed/refractory MM in 2003, we have seen considerable improvement in extending MM patient survival. However, most
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Nutrients, 13(8) (2021-08-28)
l-Arginine is an important nutrient in the infant diet that significantly regulates the maturation of the immune system in neonates, including the maturation of CD4+ T cells. The biological activities of CD4+ T cells differ substantially between neonates and adults
Adele Goldman-Pinkovich et al.
mBio, 11(5) (2020-10-15)
Arginine homeostasis in lysosomes is critical for the growth and metabolism of mammalian cells. Phagolysosomes of macrophages are the niche where the parasitic protozoan Leishmania resides and causes human leishmaniasis. During infection, parasites encounter arginine deprivation, which is monitored by
Rachel S Lee et al.
Neoplasia (New York, N.Y.), 21(4), 389-400 (2019-03-23)
Approximately 50% of prostate cancers harbor the TMPRSS2:ERG fusion, resulting in elevated expression of the ERG transcription factor. Despite the identification of this subclass of prostate cancers, no personalized therapeutic strategies have achieved clinical implementation. Kinases are attractive therapeutic targets
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Clinical cancer research : an official journal of the American Association for Cancer Research, 22(21), 5383-5393 (2016-11-03)
This study was performed to further our understanding of the biological and genetic basis of follicular lymphoma and to identify potential novel therapy targets. We analyzed previously generated whole exome sequencing data of 23 follicular lymphoma cases and one transformed

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