R3025
Anti-Rho-GDI antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution, ~1 mg/mL
别名:
Anti-GDIA1, Anti-HEL-S-47e, Anti-NPHS8, Anti-RHOGDI, Anti-RHOGDI-1
产品名称
Anti-Rho-GDI antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution, ~1 mg/mL
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~28 kDa
species reactivity
mouse, bovine, human
concentration
~1 mg/mL
technique(s)
immunoprecipitation (IP): 4 μg using 500 μg of Jurkat RIPA lysate
western blot: 0.5-1.0 μg/mL using RIPA lysates from human HL-60, HeLa, Hs294T, Jurkat and murine 3T3/NIH cells and mouse 3T3/A31
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ARHGDIA(396)
mouse ... Arhgdia(192662)
Application
Anti-Rho-GDI antibody produced in rabbit has been used in immunoblotting.
Anti-Rho-GDI has been used in western blotting.
Biochem/physiol Actions
Rho proteins control the actin and microtubule cytoskeleton. RhoGDIα is necessary for the spatial and temporal modulation of RhoGNBPs (guanine nucleotide-binding proteins). In HCC (hepatocellular carcinoma) patients, absence of ARHGDIA (Rho guanine nucleotide dissociation inhibitor) affects the prognosis and induces invasion and metastasis of HCC cells.
The antibody recognizes Rho-GDI and cross-reacts with D4-GDI.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Rho guanine nucleotide dissociation inhibitor I (RhoGDI), also known as Rho GDP dissociation inhibitor α (ARHGDIA) is encoded by the gene mapped to human chromosome 17q25.3-25-3. RhoGDI is the member of Rho/Rac protein family. The encoded protein consists of 204 amino acids and has a molecular mass of 23,421,000 Mr. In mammals, three RhoGDI isoforms exists: RhoGDIα, RhoGDIβ and RhoGDIγ. RhoGDIα is widely expressed. RhoGDIβ is mainly expressed in hematopoietic cells and RhoGDIγ is present in the brain, lung, kidney and testis.
Rho proteins are basically described as guanine nucleotide-binding proteins (GNBPs). RhoGDI (Rho guanine nucleotide dissociation inhibitor)/ARHGDIA (Rho guanine nucleotide dissociation inhibitor) has three subtypes namely- RhoGDIα (Rho GDP dissociation inhibitor α), RhoGDIβ (Rho GDP dissociation inhibitor β) and RhoGDIγ (Rho GDP dissociation inhibitor γ). RhoGDIα is expressed everywhere. It is located on human chromosome 17q25.3. Its protein has 204 amino acids and belongs to the Rho/Rac family.
Immunogen
GST fusion protein corresponding to full-length human Rho-GDI.
Physical form
Solution in 0.1 M Tris-glycine, pH 7.4, containing 0.15 M NaCl and 0.05% sodium azide.
Preparation Note
Purified using protein A
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
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Pediaditakis I, et al.
Frontiers in Pharmacology, 7, 512-512 (2016)
Rac signaling in breast cancer: a tale of GEFs and GAPs
Wertheimer E, et al.
Cellular Signalling, 24(2), 353-362 (2012)
Structural and Mechanistic Insights into the Regulation of the Fundamental Rho Regulator RhoGDI? by Lysine Acetylation
Kuhlmann N, et al.
The Journal of Biological Chemistry, 291 (2016)
Jing Sun et al.
Science signaling, 9(409), ra3-ra3 (2016-01-07)
Toll-like receptors (TLRs) are a major class of pattern recognition receptors, which mediate the responses of innate immune cells to microbial stimuli. To systematically determine the roles of proteins in canonical TLR signaling pathways, we conducted an RNA interference (RNAi)-based
Kyu-Seon Oh et al.
Immunity, 47(2), 298-309 (2017-08-13)
Despite the widespread use of glucocorticoids (GCs), their anti-inflammatory effects are not understood mechanistically. Numerous investigations have examined the effects of glucocorticoid receptor (GR) activation prior to inflammatory challenges. However, clinical situations are emulated by a GC intervention initiated in
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