R3653
Anti-Radixin antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
别名:
Anti-DFNB24
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关于此项目
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IP, WB
Citations:
20
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~80 kDa
species reactivity
mouse, rat, human
technique(s)
immunoprecipitation (IP): 10-20 μg using RIPA extract (250 μg) of rat liver, indirect immunofluorescence: 10-20 μg/mL using cultured human HeLa cells, western blot: 1-2 μg/mL using whole extract of cultured HeLa cells, western blot: 1-2 μg/mL using whole extract of cultured mouse NIH3T3 cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... RDX(5962)
mouse ... Rdx(19684)
rat ... Rdx(315655)
General description
RDX (radixin) belongs to the ERM (ezrin-radixin-moesin) family. It is present in several normal cells, neoplastic cells, epithelial and lymphoid cells. It is located on chromosome 11. In mouse, RDX is found in the hair cell stereocilia of inner ear.
Immunogen
synthetic peptide corresponding to amino acid residues 400-409 of human radixin with an N-terminal added tripeptide, conjugated to KLH. The corresponding sequence is identical in mouse, rat, and pig and differs by two amino acids in chicken.
Application
Anti-Radixin antibody produced in rabbit has been used in
- immunoblotting
- immunoprecipitation
- immunofluorescence staining of cultured cells
Biochem/physiol Actions
In human, knockdown of radixin (RDX) represses the development of glioblastoma cell in vitro and in vivo. It participates in cell motility, invasion and tumor progression. It acts as a membrane-cytoskeletal linker in cell surface structures that are abundant with actin. It is necessary for the arrangement of cortical cytoskeleton. RDX mutation can lead to DFNB24 hearing loss in human.
Radixin is the dominant ezrin-radixin-moesin (ERM) protein in liver bile canaliculi where it is considered to have a critical role in bile conjugated-bilirubin secretion by influencing the cellular localization of the multidrug resistance protein 2 (MRP2). Resting normal human blood cells lack detectable radixin. Radixin is found in classical NK cells. Radixin has closed and open forms corresponding to the inactive and active forms as cross-linkers between actin filaments. It may be phosphorylated and it plays an important role in the activation of the Rho family members by recruiting their positive and negative regulators.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
Radixin regulates cell migration and cell-cell adhesion through Rac1.
Valderrama F, et al.
Journal of Cell Science, 125(14), 3310-3319 (2012)
Differential expression and distribution of ezrin, radixin and moesin in human natural killer cells.
Ramoni C, et al.
European Journal of Immunology, 32(11), 3059-3065 (2002)
Staurosporine-induced collapse of cochlear hair bundles
Goodyear RJ, et al.
The Journal of Comparative Neurology, 522(14), 3281-3294 (2014)
Structural conversion between open and closed forms of radixin: low-angle shadowing electron microscopy.
Ishikawa H, et al.
Journal of molecular biology, 310(5), 973-978 (2001)
Radixin knockdown by RNA interference suppresses human glioblastoma cell growth in vitro and in vivo
Qin JJ, et al.
Asian Pacific Journal of Cancer Prevention, 15(22), 9805-9812 (2014)
相关内容
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