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Molecular Biology
form
lyophilized powder
mol wt
25 kDa
solubility
water: 2 mg/mL
storage temp.
2-8°C
General description
转移 RNA 是从啤酒酵母中提取的。该转移 RNA 对氨基酸苯丙氨酸的转移具有特异性。
100% 纯 tRNA 的苯丙氨酸受体活性为每 A260 单位 1800 pmole。该编号与批次特定分析的比较可用于指示产品纯度。进一步纯化 tRNA 的方法已经发表。
本产品的含量测定基于已发表的方法。用苯丙氨酰转移核糖核酸合成酶的粗制剂测定活性。苯丙氨酸特异性 tRNA 在试验中用作酶的底物。
本品不适合用作载体 tRNA，以帮助乙醇沉淀极少量的 DNA。产品编号。推荐将 R 8508 和 R 5636 用作 DNA 纯化和沉淀的载体 tRNA。
100% 纯 tRNA 的苯丙氨酸受体活性为每 A260 单位 1800 pmole。该编号与批次特定分析的比较可用于指示产品纯度。进一步纯化 tRNA 的方法已经发表。
本产品的含量测定基于已发表的方法。用苯丙氨酰转移核糖核酸合成酶的粗制剂测定活性。苯丙氨酸特异性 tRNA 在试验中用作酶的底物。
本品不适合用作载体 tRNA，以帮助乙醇沉淀极少量的 DNA。产品编号。推荐将 R 8508 和 R 5636 用作 DNA 纯化和沉淀的载体 tRNA。
Application
适用于：
- 作为 tRNA 标准品
- tRNA 合成酶试验中作为底物
- 在涉及 tRNA 的其他反应中作为底物
Preparation Note
Wimmer,E. 等人方法制备，Biochemistry，7, 2623 (1968).
Other Notes
tRNA 以冻干粉形式提供，可溶于水，浓度为 2 mg/mL。
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Mariana Pavon-Eternod et al.
Nucleic acids research, 37(21), 7268-7280 (2009-09-29)
Increased proliferation and elevated levels of protein synthesis are characteristics of transformed and tumor cells. Though components of the translation machinery are often misregulated in cancers, what role tRNA plays in cancer cells has not been explored. We compare genome-wide
W Yan et al.
The EMBO journal, 17(16), 4809-4817 (1998-08-26)
Correct folding of newly synthesized polypeptides is thought to be facilitated by Hsp70 molecular chaperones in conjunction with DnaJ cohort proteins. In Saccharomyces cerevisiae, SSB proteins are ribosome-associated Hsp70s which interact with the newly synthesized nascent polypeptide chain. Here we
Tissue-specific differences in human transfer RNA expression.
Dittmar, K.A., et al.
PLoS Genetics, 2, 2107-2107 (2006)
Noa Aharon-Hefetz et al.
eLife, 9 (2020-12-29)
Different subsets of the tRNA pool in human cells are expressed in different cellular conditions. The 'proliferation-tRNAs' are induced upon normal and cancerous cell division, while the 'differentiation-tRNAs' are active in non-dividing, differentiated cells. Here we examine the essentiality of
Andrey Chursov et al.
Nucleic acids research, 41(Web Server issue), W486-W491 (2013-06-15)
Although multiple biological phenomena are related to temperature (e.g. elevation of body temperature due to an illness, adaptation to environmental temperature conditions, biology of coldblooded versus warm-blooded organisms), the molecular mechanisms of these processes remain to be understood. Perturbations of
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