质量水平
表单
powder
技术
cell culture | mammalian: suitable
组分
L-glutamine: yes
NaHCO3: no
phenol red: yes
HEPES: 25 mM
sodium pyruvate: no
运输
ambient
储存温度
2-8°C
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一般描述
RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成，用于在悬浮培养中支持淋巴母细胞生长，但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用，但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。
制备说明
按照每升培养基16.4克粉末配制。
补充2.0 g/L碳酸氢钠。
储存分类代码
11 - Combustible Solids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Tayebeh Mehdipour et al.
Immunology, 159(1), 96-108 (2019-10-10)
Among various solid tumours, gastric cancer (GC) is one of the leading causes of cancer-related deaths worldwide. Expansion into the peritoneal cavity, which results from dissemination of diffuse cancer cells, is the main cause of mortality in gastric adenocarcinoma patients.
Phosphorylation of Toxoplasma gondii Secreted Proteins during Acute and Chronic Stages of Infection.
Joanna C Young et al.
mSphere, 5(5) (2020-09-11)
The intracellular parasite Toxoplasma gondii resides within a membrane-bound parasitophorous vacuole (PV) and secretes an array of proteins to establish this replicative niche. It has been shown previously that Toxoplasma secretes kinases and that numerous proteins are phosphorylated after secretion.
Jayant Dewangan et al.
Apoptosis : an international journal on programmed cell death, 22(10), 1246-1259 (2017-07-28)
Resveratrol (RES) is a natural polyphenol having anti-proliferative activity against breast cancer cells. RES in combination with other chemo modulatory agents, minimizes toxicity and increases efficacy of the treatment. Salinomycin (SAL), a monocarboxylic polyether ionophore is known for selectively targeting
Shilpee Sharma et al.
The Journal of biological chemistry, 293(30), 11687-11708 (2018-05-19)
HIV-1 subtype C (HIV-1C) may duplicate longer amino acid stretches in the p6 Gag protein, leading to the creation of an additional Pro-Thr/Ser-Ala-Pro (PTAP) motif necessary for viral packaging. However, the biological significance of a duplication of the PTAP motif
Fhernanda R Smiderle et al.
BMC complementary and alternative medicine, 11, 58-58 (2011-07-27)
Mushroom polysaccharides have traditionally been used for the prevention and treatment of a multitude of disorders like infectious illnesses, cancers and various autoimmune diseases. Crude mushroom extracts have been tested without detailed chemical analyses of its polysaccharide content. For the
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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