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一般描述
Sigma′定量聚合酶链反应（qPCR）参考染料是一种专有染料，用于实时PCR。在使用SYBR绿、分子探针或双标记探针化学进行实时检测时，它可以用于反应数据的标准化。 我们提供100×参考染料，最大激发和放射峰分别出现在586nm和605nm。ROX参考染料仪器设定符合qPCR参考染料检测。
应用
定量 PCR 参考染料已被用于:
- 制备用于实时定量聚合酶链反应 （RT-qPCR）的预混液
- 作为反应混合物的成分，用于通过定量聚合酶链式反应（qPCR）检测艰难梭菌
- 通过 qPCR 分析细胞 DNA 污染程度
其他说明
定量PCR参考染料仅用于研发目的。不可用于药物、家庭或其他用途。
警示用语：
Warning
危险分类
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves
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Sambrook, J. et al.
Molecular Cloning: A Laboratory Manual, 3rd (2000)
商品
Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.
实验方案
Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.
我们的SYBR Green qPCR实验方案是一种旨在准确定量基因表达和RT-PCR反应的的方法
Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions
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