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Merck
CN

R4526

Sigma-Aldrich

定量PCR参考染料

100 ×, solution

别名:

qPCR参考染料, ROX

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关于此项目

UNSPSC代码:
41106300
NACRES:
NA.55
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表单

solution

质量水平

用途

sufficient for ≥600 reactions

浓度

100 ×

技术

PCR: suitable

颜色

red

溶解性

water: soluble

储存温度

2-8°C

一般描述

Sigma′定量聚合酶链反应(qPCR)参考染料是一种专有染料,用于实时PCR。在使用SYBR绿、分子探针或双标记探针化学进行实时检测时,它可以用于反应数据的标准化。 我们提供100×参考染料,最大激发和放射峰分别出现在586nm和605nm。ROX参考染料仪器设定符合qPCR参考染料检测。

应用

定量 PCR 参考染料已被用于:
  • 制备用于实时定量聚合酶链反应 (RT-qPCR)的预混液
  • 作为反应混合物的成分,用于通过定量聚合酶链式反应(qPCR)检测艰难梭菌
  • 通过 qPCR 分析细胞 DNA 污染程度

其他说明

定量PCR参考染料仅用于研发目的。不可用于药物、家庭或其他用途。

象形图

Exclamation markEnvironment

警示用语:

Warning

危险分类

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Ryan Manow et al.
Journal of industrial microbiology & biotechnology, 39(7), 977-985 (2012-03-01)
Previously, a native homoethanol pathway was engineered in Escherichia coli B by deletions of competing pathway genes and anaerobic expression of pyruvate dehydrogenase (PDH encoded by aceEF-lpd). The resulting ethanol pathway involves glycolysis, PDH, and alcohol dehydrogenase (AdhE). The E.
Ryan Manow et al.
World journal of microbiology & biotechnology, 36(4), 59-59 (2020-04-03)
An endogenous homoethanol pathway (glucose/1.2 xylose => 2 pyruvate => 2 ethanol) was previously engineered in Escherichia coli SZ410 via eliminating acid-producing pathways and anaerobic expression of the pyruvate dehydrogenase complex (aceEF-lpd operon). This ethanologenic derivative was subsequently engineered through adaptive evolution and partial
Kevin Antoine Brown et al.
Infection control and hospital epidemiology, 39(8), 917-923 (2018-08-10)
Clostridium difficile spores play an important role in transmission and can survive in the environment for several months. Optimal methods for measuring environmental C. difficile are unknown. We sought to determine whether increased sample surface area improved detection of C.
Increased environmental sample area and recovery of Clostridium difficile spores from hospital surfaces by quantitative PCR and enrichment culture
Brown KA, et al.
Infection Control and Hospital Epidemiology : The Official Journal of the Society of Hospital Epidemiologists of America, 39(8), 917-923 (2018)
Partial deletion of rng (RNase G)-enhanced homoethanol fermentation of xylose by the non-transgenic Escherichia coli RM10
Manow R, et al.
Journal of Industrial Microbiology & Biotechnology, 39(7), 977-985 (2012)

商品

Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.

实验方案

Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.

我们的 SYBR Green qPCR 协议是专为检测基因表达和 RT-PCR 反应的精确定量而设计的方法。

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions

我们的 SYBR Green qPCR 协议是专为检测基因表达和 RT-PCR 反应的精确定量而设计的方法。

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